CYCLINs are regulatory proteins active for only portions of the cell cycle. Their transcription is activated at specific points during the cell cycle, for example, for CYCLIN1 (a CYCLIN B) of Arabidopsis during the G2 phase of the cell cycle. Since specific CYCLINs are required at different phases of the cell cycle, the stability of CYCLIN proteins is also highly regulated. This is accomplished by targeted degradation of CYCLIN proteins, also at specific times during the cell cycle, and which is mediated through a specific 9 amino acid sequence called the 'destruction box'. The CYCLIN1:GUS construct includes all the transcriptional elements required for CYCLIN1 regulation and a destruction box is translationally fused with the GUS coding sequence. How would the expression pattern you observed be altered if the destruction box was not included?
CYCLINs are regulatory proteins active for only portions of the cell cycle. Their transcription is activated at specific points during the cell cycle, for example, for CYCLIN1 (a CYCLIN B) of Arabidopsis during the G2 phase of the cell cycle. Since specific CYCLINs are required at different phases of the cell cycle, the stability of CYCLIN proteins is also highly regulated. This is accomplished by targeted degradation of CYCLIN proteins, also at specific times during the cell cycle, and which is mediated through a specific 9 amino acid sequence called the 'destruction box'. The CYCLIN1:GUS construct includes all the transcriptional elements required for CYCLIN1 regulation and a destruction box is translationally fused with the GUS coding sequence. How would the expression pattern you observed be altered if the destruction box was not included?
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