Lane 1: No proteins added Lane 2: TFIID Lane 3: TFIIB Lane 4: RNA polymerase Il Lane 5: TFIID + TEIIB Lane 6: TFIID + RNA 1 2 3 4 5 6 7 polymerase II Lane 7: TFIID + TFIIB + RNA polymerase I| 1100 bp
Gene Interactions
When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
An electrophoretic mobility shift assay can be used to study the binding
of proteins to a segment of DNA. In the results shown here, an
electrophoretic mobility shift assay was used to examine the requirements
for the binding of RNA polymerase II (from eukaryotic cells)
to the promoter of a protein-encoding gene. The assembly of general
transcription factors and RNA polymerase II at the core promoter
is described in Chapter 14 (Figure 14.14). In this experiment, the
segment of DNA containing a promoter sequence was 1100 bp
in length. The fragment was mixed with various combinations of
proteins
and then subjected to an electrophoretic mobility shift assay.
Explain which proteins (TFIID, TFIIB, or RNA polymerase II) are
able to bind to this DNA fragment by themselves. Which transcription
factors (i.e., TFIID or TFIIB) are needed for the binding of
RNA polymerase II?
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