A scientist seeks to synthesize a blood clotting factor protein in lab. To perform this experiment, she utilizes recombinant DNA technology where she inserts the gene of interest encoding for the particular protein from a eukaryotic cell into bacterial plasmids. These plasmids are these transformed into E.coli for expression. During structural elucidation of the product, she is disappointed to discover that the gene product from the bacteria is not the correct protein. What corrective step could she take in the procedure to fix this issue? Justify your answer.

Q: Antibiotic-resistance is one of the gravest dangers to the world’s medical system. As a result, Dr.…

A: Antibiotic resistance refers to the ability of bacteria to resist the effects of antibiotics, making…

Q: You are studying a human gene, and try to express the protein in E. coli bacterial cells. To do…

A: Cloning is the process of creating a genetically identical copy of a biological entity. Cloning can…

Q: Northern blots are valuable tools to analyze the mRNA level present in a sample. Describe an…

A: The goal of a northern blot is to monitor the expression of a particular genome in tissues, organs,…

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A: Cancer is a fatal disease that has both physical and mental consequences for a person. Cancer can…

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Q: The data below depicts the binding of the E coli RNA Polymerase to DNA containing a promoter…

A: RNA polymerase is specific enzyme that synthesized RNA from the strand of DNA. They helps to copying…

Q: UP elements Select an answer and submit. For keyboard navigation, use the up/down arrow keys to…

A: Answer. A promoter can be defined as the cis-acting, position-dependent DNA sequence necessary for…

Q: A full-length eukaryotic gene is inserted into a bacterial chromosome. The gene contains a complete…

A: The eukaryotic DNA is composed of coding and non-coding sequences, almost 90% are non-coding…

Q: Give the result(s) of the experiment in "MicroRNAs Control De Novo DNA Methylation Through…

A: INTRODUCTION MicroRNAs (miRNAs) are a class of small, single-stranded non-coding RNAs that regulate…

Q: sRNA acts as a marker, flagging the inhibitory protein for ubiquitination and allowing translation…

A: Proteins are synthesized by the process of translation and are modified after post-translational…

Q: A gene product of unknown function was subjected to both two hybrid analysis and BioID. In the two…

A: Two-hybrid analysis is a molecular biology technique used to discover protein-protein interactions…

Q: e previous problem you proposed a model for how this gene could be regulated. Suppose that you carry…

A: If the mutation is recessive mutant then there should be mutation in both allele to see this effect.…

Q: Here is a list of knockout gene mutations that are desired to make in E. coli genome. You should…

A: A number of knockout gene mutations in the E. coli genome are shown to us in this scenario. Each…

Q: During experimental RNAi, how does the researcher affect expression of a target gene? Group of…

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A: Yeast is a eukaryotic organism that possesses a network of internal membranes to sort proteins to a…

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Q: Suppose that an investigative team conducted an RNA-Seq experiment on mouse liver cells. They found…

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Q: Numerous diseases are treated by injection of polypeptide growth factors, such as human growth…

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Q: Which of the following statements about the attempt to express a eukaryotic gene in bacteria is…

A: The regulation of gene expression in eukaryotes occurs at several level, which is far more diverse…

Q: Which of the following statements is not true about the guide RNA (see this interactive…

A: The objective of the question is to identify the incorrect statement about guide RNA in the context…

Q: How to incorporate prokaryotic gene into eukaryotic cell to make an transgenic organism?In this case…

A: Transformation is a technique used to incorporate foreign DNA into a target organism. In this case,…

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A: Protein enrichment is a method by which the proteins of a specific type present in the sample are…

Q: To create a cDNA library, cDNA can be inserted into vectors and cloned. In the analysis of cDNA…

A: A gene is an area of DNA molecule or polymer of amino acid that encodes function. A chromosome…

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A: When lactose is not present in the medium operon does not transcribe because of the presence of lac…

Q: Prior to the publication of the article from which this assay comes from (picture), the Cas9…

A: In the graphical representation of SDS-PAGE analysis, separate bands corresponding to various Cas9…

Q: In the RPO7 mutant lane, what is your interpretation of the results? Transcription of the 30S rRNA…

A: There are a few important terms:Northern blotting:  It is the molecular biology technique that makes…

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A: Biotechnology is the use of our understanding of biological processes to develop useful applications…

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Q: RNA are extracted from liver cells and separated in agarose gel by electrophoresis side-by-side with…

A: Biotechnology is the part of applied science that utilizations living organic entities and their…

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A: Cancer results from the uncontrolled cell division giving rise to a lump of cells that may be…

Q: A partial diploid in E. coli is created so that LacI is no longer expressed from the genome and is…

A: Partial diploid E.coli has both chromosomal DNA (i.e. genome) and plasmid DNA, but all the genes are…

Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.