In the two competition study experiments below, what can you say about the relative affinities of the labeled ligand and the unlabeled competitor ligand? Remember to check the concentrations of both in each experiment. extracellular plasma membrane intracellular Competition study Competitor unlabeled ligand Labeled ligand †††† Receptor 10 nM Competitor unlabeled ligand 10 nM Labeled ligand †††Ÿ 9 nM Competitor unlabeled ligand 3 nM Labeled ligand

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Q: -Inhibitor +Inhibitor [S] (mM) V&ν βσπ:(μmol/sec) ν0&νβσπ: &νβσπ: (μmol/sec) 0.0001 33 17 0.0005 71…

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Q: Ligand Y for mouse Y for rat Y for human concentration receptor receptor receptor (nM) 0.20 0.048…

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Q: VA 256 192 128 9 1.5 10- 38 25- 19 125- 64. .032- .023 .047

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