The table below summarizes the results of three kinetic experiments using the same enzyme and substrate. The same substrate concentrations were used for each experiment (column 1). The second column is the measured velocities for the control experiment, and the last columns are data collected in the presence of two different inhibitors, X and z. [S] Control +Inhibitor X +Inhibitor Z (mM) Vo (mM/s) Vo (mM/s) Vo (mM/s) 1.0 2.50 1.17 0.77 2.0 4.00 2.10 1.25 5.0 6.30 4.00 2.00 10.0 7.60 5.70 2.50 20.0 9.00 7.20 2.86
The table below summarizes the results of three kinetic experiments using the same enzyme and substrate. The same substrate concentrations were used for each experiment (column 1). The second column is the measured velocities for the control experiment, and the last columns are data collected in the presence of two different inhibitors, X and z. [S] Control +Inhibitor X +Inhibitor Z (mM) Vo (mM/s) Vo (mM/s) Vo (mM/s) 1.0 2.50 1.17 0.77 2.0 4.00 2.10 1.25 5.0 6.30 4.00 2.00 10.0 7.60 5.70 2.50 20.0 9.00 7.20 2.86
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
Using the attachment, Answer the following questions:
Prepare a double reciprocal plot with all three experiments (lines) on the same graph. Use your graph, and then answer items 2, 3, and 4 below.
1. Calculate the Vmax or apparent Vmax for all three sets of data. Likewise, calculate the Km or apparent Km for each set.
2. For Inhibitor X, what is the mode/type of inhibition?
3. For Inhibitor Z, what is the mode/type of inhibition? By comparison of the apparent Vmax to the control Vmax, what is the value of α’, as defined in class? If Ki’ = 10 mM for this inhibitor, then what must the inhibitor concentration [Z] be?
![The table below summarizes the results of three kinetic experiments using the same
enzyme and substrate. The same substrate concentrations were used for each
experiment (column 1). The second column is the measured velocities for the control
experiment, and the last columns are data collected in the presence of two different
inhibitors, X and Z.
[S]
Control
+Inhibitor X +Inhibitor Z
(mM)
Vo (mM/s)
Vo (mM/s)
Vo (mM/s)
1.0
2.50
1.17
0.77
2.0
4.00
2.10
1.25
5.0
6.30
4.00
2.00
10.0
7.60
5.70
2.50
20.0
9.00
7.20
2.86](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F5038e9fe-a67b-4654-bcd7-3337a3eb6f7a%2F2577ede1-e4a4-42fc-afe6-fc5602750a25%2Fr1pi57n_processed.jpeg&w=3840&q=75)
Transcribed Image Text:The table below summarizes the results of three kinetic experiments using the same
enzyme and substrate. The same substrate concentrations were used for each
experiment (column 1). The second column is the measured velocities for the control
experiment, and the last columns are data collected in the presence of two different
inhibitors, X and Z.
[S]
Control
+Inhibitor X +Inhibitor Z
(mM)
Vo (mM/s)
Vo (mM/s)
Vo (mM/s)
1.0
2.50
1.17
0.77
2.0
4.00
2.10
1.25
5.0
6.30
4.00
2.00
10.0
7.60
5.70
2.50
20.0
9.00
7.20
2.86
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