Following the production of membrane extracts using the non-ionic detergent Triton X-100, you analyze the membrane lysates via mass spectrometry and note a high content of cholesterol and sphingolipids. Furthermore, biochemical analysis of the lysates reveals potential kinase activity. What have you probably isolated?
Following the production of membrane extracts using the non-ionic detergent Triton X-100, you analyze the membrane lysates via mass spectrometry and note a high content of cholesterol and sphingolipids. Furthermore, biochemical analysis of the lysates reveals potential kinase activity. What have you probably isolated?
The methods for cell disruption are host-dependent, and the techniques for recovering membrane proteins are generally the same as those for recovering water-soluble proteins. Cell disruption results in the formation of a suspension of membrane fragments/vesicles containing membrane proteins as well as other components. As impurities, the solution also includes residual whole cells, soluble proteins, a wide variety of cell debris, and other substances. However, it is well noted that to extract any particular component a specific solution with the particular process is used which can make any person isolate a component that can be validated with its standardized activity.
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