construct! Which of the four constructs (Construct 1-4) below could you use to make your own mouse knockout? For each one you did not choose, explain why you did not choose that construct. Part of DHCR7 gene structure is shown on top row in the schematic. (Antibiotic selection and negative selection genes have been abbreviated.)

Q: A study by Bose and colleagues (1998. Blood 92: 3362–3367) and a previous study by Biernaux and…

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Q: 6) E. coli can use the permease encoded by Lacy to take up the sugar raffinose, however raffinose…

A: Lac operon  Lactose operon is the group of three gene lacZ, lacY, and lacA which are linked to…

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Q: Can you select answers in each bracket? CRISPR-Cas9 is a system for directly changing the sequence…

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A: Yes, Argonaute proteins are the active part of RNA-induced silencing complex, cleaving the target…

Q: - How did the following in-vitro transcription assay utilizing the rrnB promoter help elucidate the…

A: Through a direct contact with the C-terminal domain of the RNA polymerase subunit (CTD), the UP…

Q: Based on the NCBI Blastp and reciprocal analysis, are there any mutations to suggest that mus…

A: In thе contеxt of tеsting thе human FGFR3 gеnе in Mus musculus, NCBI Blastp and rеciprocal analysis…

Q: control medium that demonstrates the general growth rate of mutant candidates

A: Control Medium: This medium is used to demonstrate the general growth rate of mutant candidates in…

Q: 11) (recall) Transcription: what would be the APPROPRIATE NUCLEOTIDES (?????) in newly synthesized…

A: transcription is the process by which the dna molecules are converted into messenger rna molecule .

Q: (b) Bacteriophage 434 repressor SIN Which of the following is true regarding the interaction of the…

A: The 434 repressor is a protein that plays a key role in the regulation of gene expression in the…

Q: Below is shown an 8kb region of the human genome, with the proportion of the nucleotides that are…

A: Introduction :  Any segment of a gene that will be included in the mature RNA produced by that gene…

Q: For this lac mutant in an E. coli strain, indicate whether B-galactosidase and permease would be…

A: Bacterial genes performing similar functions are typically organized along during a linear serial…

Q: DHCR7 (7-dehydrocholesterol reductase) gene.

A: This gene encodes an enzyme that removes the C(7-8) double bond in the B ring of sterols and…

Q: CRISPR-Cas9 was first developed as a molecular tool in 2012; during the next few years, its use in…

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A: Obtain the genomic  from the mice cells that encode for the protease of interest.Fragment the…

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Q: both noncoding and coding). a. Which types of SNPs affect protein production or function for the…

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Q: Question is attached

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A: "As per the Q&A guidelines, we will provide the solution to the first question. Please repost…

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Gene Interactions

When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.

Gene Expression

Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.