CRISPR-Cas9 was first developed as a molecular tool in 2012; during the next few years, its use in molecular biology exploded, as scientists around the world began applying it to many different research problems, and hundreds of research papers describing its application were published. Explain why CRISPR-Cas is such a powerful tool in molecular genetics.

Q: Discuss how CRISPR-cas functions as a genome editing tool.

A: Genome editing:It is also known as gene editing or genome engineering. It is a type of genetic…

Q: Describe the following techniques for determining gene or protein function with advantages…

A:

Q: If a pair of 10-mer primers are used in a PCR, what would be the number of expected binding sites in…

A: PCR is a polymerase chain reaction, in this process DNA is amplified . For this amplification,…

Q: What are the use and the advantages of discovering CRISPR Cas-9 of Jennifer Anne Doudna in research…

A: Deoxyribonucleic acid or DNA is a nucleic acid that composed of two polynucleotide chains running in…

Q: Besides the great potential of gene editing, what are the biggest risks?  How do scientist test to…

A: Gene editing is a rapidly advancing field of genetic engineering that allows for precise…

Q: List three possible uses of site-directed mutagenesis

A: BASIC INFORMATION MUTATION  It is sudden or discontinuous variation These changes occurs in the…

Q: For a coding gene, purifying (negative) selection will result in more synonymous (silent) site…

A: Purifying selection is the most predominant form of natural selection or negative selection. As it…

Q: You are interested in amplifying the putative gene encoding the 480-bp protein TMR (Total Memory…

A: We are given with the gene that is needed to be amplified by PCR. The image shows a region of DNA…

Q: 1、You have a 250 bp DNA sequence from a human protein-coding gene.  What's the best tool to find out…

A: When analyzing DNA sequences and performing tasks such as gene identification and sequence…

Q: A graduate student studying yfgA expression in the liver and heart performs an RNA-seq experiment.…

A: In order for a gene to produce functional gene products, such as proteins or non-coding RNA, and…

Q: When a genome is edited using the CRISPR/Cas9 system, a knockout allele can be produced if DNA is…

A: CRISPR: CRISPR  is a newly emerging tool that is used by scientists to alter the genome structure by…

Q: How the amplification of other genomic methods such as optical mapping helps to overcome the…

A: The amplification of other genomic methods like optical mapping  has definitely improved the…

Q: Make a list of possible research questions that could be answeredusing site-directed mutagenesis or…

A: Site-directed mutagenesis refers to an in-vitro or artificial technique with the help of which…

Q: What does CRISPR-Cas stand for and how does it work? What are the molecules involved with CRISPR-Cas

A: Introduction Genome editing:- It is also known as gene editing or genome engineering. It is a type…

Q: Previous research had found odd sequences, spacers, and repeats, within some bacterial genomes.…

A: It is the spacers that enable the adaptable and gene-specific inactivating mechanism of the CRISPR…

Q: Prior to the publication of the article from which this assay comes from (picture), the Cas9…

A: SDS-PAGE Analysis of Cas9 Orthologsa. Experimental Question and HypothesisResearch Question: The…

Q: ain A: wild type........ .CT value of 15 ain B: has mutation X in the promoter region........CT…

A: Quantitative reverse transcription polymerase chain reaction, also called RT-qPCR, is a molecular…

Q: What is the Guide RNA (gRNA) a chimera of? Why use a gRNA?

A: Disclaimer: "According to the Bartleby guidelines, only the first question is to be answered first…

Q: Why the sequence alignment is extremely important in designing a construct for developing a…

A: Sequence alignment It is defined as the way of arranging the sequences of DNA, RNA or protein to…

Q: occurring

A: The Department of agriculture regulates field testing of GM crops for research. The environmental…

Q: What is it about the Cas9 protein that makes it an efficient DNAediting tool?

A: DNA and RNA are the genetic elements present in almost all cells. DNA encodes for proteins through…

Q: Below is shown an 8kb region of the human genome, with the proportion of the nucleotides that are…

A: Introduction :  Any segment of a gene that will be included in the mature RNA produced by that gene…

Q: Some synthetic biologists have proposed creating an entirely new, freeliving organism with a minimal…

A: A genome is the entire DNA collection of an organism, including all genes. All the details needed to…

Q: Briefly, how can we have so many TCRs and BCRs (billions!) with only 100 genes dedicated to making…

A: The T-cell receptor (TCR) is a protein complex present on the exterior of T cells, also known as T…

Q: What are potential uses of CRISPR-Cas9 Technologies? Silence genes to study their function Identify…

A: CRISPR-Cas9 is a gene editing tool that allows scientists to precisely target and cut specific DNA…

Q: (2816) Xmnl (2697) Scal (2365) Nm All (2287) Bpml (2278) Bsal (2217) Andl Pvu 1 (3735) Ssp 1 (4170)…

A: The SV40 genome is a circular double stranded DNA organized in a single replicon. The process of…

Q: the most efficient general strategy for whole genome sequencing is ? (a) double the coding sequence…

A: Whole-genome sequencing abbreviated as WGS is a method used to comprehensively analyze the entire…

Q: What has been the most significant finding of the ENCODE project?Why was the project expanded to…

A: ENCODE stands for Encyclopedia of DNA elements. It was a research project that came after the Human…

Q: What would a scientist need to use CRISPR-Cas system to introduce a specific point mutation into a…

A: When the target DNA is located, Cas9, one of the CRISPR system's enzymes, latches to it and chops…

Q: How can the efficiency of homologous recombination be improved when using CRISPR-Cas9, compared to…

A: CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) is a technique derived from…

Q: What is genetic engineering? What organisms can it be performed on? Please give an example of at…

A: * Genetic engineering is also known as gene manipulation or modification. * It is a process to alter…

Q: As we described in class, in the early 1960's Francis Crick and colleagues set out to determine how…

A: Introduction  Genetic code   The sequence of bases that encodes a functional protein molecule is…

Q: 10) Scientists have engineered a simplified CRISPR-cas system for use in mammalian cells. What were…

A: The clustered regularly interspaced short palindromic repeats (CRISPR) is a technology that is used…

Q: What is Central Dogma of Molecular Biology and how this concept is used in the development of…

A: Genetically modified organisms are those that have been modified by humans in order to show those…

Q: Genome annotation refers to ... 1.) lining up overlapping regions in short shotgun sequencing reads…

A: Introduction:- Genome consists of all the genetic and biological information of an individual…

Q: i) Suppose we want to insert the GFP sequence after the promoter of a gene X to create a fusion…

A: The CRISPR Cas9 system is a tool for cutting a DNA at a particular location. This system has 2…

Q: Your colleague wants to align the complete sequence of the collagen gene from humans to the…

A: Homologous genes are genes that share a common ancestry. When two species share a common ancestor,…

Q: Your insert has the sequence TGGATCCTTATAATGTTT.....Which pET-41 vector will you use to clone with…

A: To determine which vector is best for you, you will need to consider the reading frame of your…

Q: Now you have the gene sequence. Now you would like to clone it into an expression vector to grow up…

A: Answer. The majority of eukaryotic genes contain non-coding DNA. The genes tend to have a split…

Genomic studies

Genomic studies (genomics) is the study of the whole genome of an organism. Using bioinformatics and high-performance computing, genomic researchers examined massive amounts of deoxyribonucleic acid (DNA)-sequence data to discover variations or mutations that affect drug response, health, or disease. Genomics is a much younger discipline than genetics, having only emerged in the last few decades as a result of technological advancements in DNA sequencing and computational biology.