Genome annotation refers to ... 1.) lining up overlapping regions in short shotgun sequencing reads to assemble larger contiguous DNA sequences (contigs/scaffolds). 2.) using long-read sequencing platforms (such as PacBio or Oxford Nanopore) to gather information about the epigenetic status of each region of a sequenced genome. 3.) the process of predicting which parts of a genome sequence code for functional products (such as protein-coding genes), what those products do, and assigning them names. 4.) sequencing messengeRNA measure the relative expression levels of genes in one or more tissue samples.

Q: Does one have a practical idea of the role of CRISPR/Cas9 in genome editing via cutting of DNA?

A: A molecular process in which some discontinuous change to nucleotide sequences in their genomic DNA…

Q: What is the main reason for using a cDNA library rather than a genomic library to isolate a human…

A: Introduction :- cDNA stands for "complementary DNA". It is a type of DNA that is synthesized from…

Q: What is an advantage of making a cDNA library rather than a genomic library?

A: DNA library or the gene library is the collection of DNA sequences from different organisms. These…

Q: organisms

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Q: For a coding gene, purifying (negative) selection will result in more synonymous (silent) site…

A: Purifying selection is the most predominant form of natural selection or negative selection. As it…

Q: You are interested in amplifying the putative gene encoding the 480-bp protein TMR (Total Memory…

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Q: The goal of most gene therapies is to insert a healthy copy of a gene into the genome.  Besides…

A: Genome editing:It is also known as gene editing or genome engineering. It is a type of genetic…

Q: You obtained the sequence of the frog gene X you amplified in Question #16 through a process called…

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Q: Which of the following is not true of cDNA produced usinghuman brain tissue as the starting…

A: Introduction  Central Dogma: it is the key mechanism by which DNA can be transcribed into mRNA by…

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Q: Site directed mutagenesis is used to: 1.determine the critical base per sequences in a Genome 2.…

A: DNA consists of double strands of sequentially arranged nucleotides. The nucleotide sequence present…

Q: You are trying to study the effects of Drug A on the expression of Gene X in a tumor sample (lane…

A: Reverse transcription enzyme chain reaction (RT-PCR) may be a variation of normal PCR that involves…

Q: the most efficient general strategy for whole genome sequencing is ? (a) double the coding sequence…

A: Whole-genome sequencing abbreviated as WGS is a method used to comprehensively analyze the entire…

Q: Practical applications of recombinant DNA technology include the: 1. Efficient production of useful…

A: Recombinant DNA technology, also known as genetic engineering, is a technique used to manipulate and…

Q: Define `transcriptome'.  Briefly describe the genome tiling array technology to `visualize'…

A: Hi dear, here's your answer what you want.can you please give me a like for this answer. * Every one…

Q: Check all the statements that are TRUE regarding 16S or ITS (microbiome) illumina (NGS) sequencing…

A: The Illumina sequencing chemistry is extremely precise, with the great majority of bases scoring Q30…

Q: CRISPR/Cas9 can be used in genome editing. Among the following statements, which one is correct?…

A: Bacteria have evolved various mechanism to resist against the viruses like bacteriophage some of…

Q: assess anatomical distribution of gene expression.   genomic pcr nortbern blotting Rnai in situ…

A: The genes are the functional part of the DNA that undergoes transcriptional process and ultimately…

Q: Your insert has the sequence TGGATCCTTATAATGTTT.....Which pET-41 vector will you use to clone with…

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Q: A number of advances have been made in biotechnology. CRISPR/Cas9 one of the most controversial, and…

A: CRISPR/Cas9 is found in the prokaryote system Cas 9 is an enzyme that cut DNA

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Q: You would expect to find a sequence corresponding to the 5' untranslated region (5'-UTR) of a gene…

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Q: Some people have argued that editing the genomes of human embryos is ethically defensible as long as…

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Q: Now you have the gene sequence. Now you would like to clone it into an expression vector to grow up…

A: Answer. The majority of eukaryotic genes contain non-coding DNA. The genes tend to have a split…

Q: CRISPR/Cas9 mediated genome editing has provided an unparalleled means of manipulating the genome.…

A: CRISPR/Cas9 (Clustered regularly interspaced short palindromic repeats/CRISPR-associated genes) is a…

Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.