Can you select answers in each bracket? CRISPR-Cas9 is a system for directly changing the sequence of [ Select ] ["DNA", "RNA"] and is derived from bacterial systems designed to block infection by [ Select ] ["bacteriophage", "HIV"] . The original system contained two RNA molecules, crRNA and tracrRNA, that formed a complex that recruited Cas9 protein. Dr. Doudna and her colleague Dr. Emmanuelle Charpentier found they could make a single guide RNA (sgRNA) that had an [ Select ] ["intramolecular interaction (H-bonding)", "RNAse activity"] that could bind and activate Cas9 to form a break at the sequence targeted by the sgRNA. While the CRISPR-Cas9 system can direct scission of the target, any change in sequence is achieved by [ Select ] ["the cells endogenous repair system", "providing cells with DNA recombination proteins"] . Therefore, generating small deletions or insertions is technically [ Select ] ["more difficult", "more feasible"] than precise replacement of a specific sequence.
Can you select answers in each bracket?
CRISPR-Cas9 is a system for directly changing the sequence of [ Select ] ["DNA", "RNA"] and is derived from bacterial systems designed to block infection by [ Select ] ["bacteriophage", "HIV"] . The original system contained two RNA molecules, crRNA and tracrRNA, that formed a complex that recruited Cas9 protein. Dr. Doudna and her colleague Dr. Emmanuelle Charpentier found they could make a single guide RNA (sgRNA) that had an [ Select ] ["intramolecular interaction (H-bonding)", "RNAse activity"] that could bind and activate Cas9 to form a break at the sequence targeted by the sgRNA. While the CRISPR-Cas9 system can direct scission of the target, any change in sequence is achieved by [ Select ] ["the cells endogenous repair system", "providing cells with DNA recombination proteins"] . Therefore, generating small deletions or insertions is technically [ Select ] ["more difficult", "more feasible"] than precise replacement of a specific sequence.
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