Circle the correct word or phrase in each bracketed region to complete a correct statement. Crispr/Cas9 genome editing depends on a(n) [tRNA / guide RNA / snRNA / DNA primer] that directs Cas9 [endonuclease / ribonuclease / protease / exonuclease] to specific sites in the genome by [binding to the major groove / PAM site recognition / binding via base complementarity]. Cas9 creates [double-stranded DNA breaks / depurination / PAM site deletion / CpG methylation] that can be repaired by [nonhomologous end joining / ligation / homology directed repair / CpG methylation] to create precise edits or [nonhomologous end joining / ligation / homology directed repair / CpG methylation] to create insertions & deletions

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Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.