Mediator is not necessary to reproduce transcription initiation reactions in vitro. If you are assembling an in vitro system to study the formation of preinitiation complex in eukaryotes and the subsequent promoter scape, which of the following mixes will work? OTFIIB-D-E-F- and H, NTPs, RNA polll, ATP, DNA template including promoter OTFIIB-D-E-F- and H, NTPs, RNA polll, DNA template including promoter

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Mediator is not necessary to reproduce transcription initiation reactions in vitro. If you are
assembling an in vitro system to study the formation of preinitiation complex in eukaryotes and the
subsequent promoter scape, which of the following mixes will work?
ο ο ο
TFIIB-D-E-F- and H, NTPs, RNA polll, ATP, DNA template including promoter
OTFIIB-D-E-F- and H, NTPs, RNA polll, DNA template including promoter
TFIIB-D-E-F- and H, dNTPs, RNA polll, ATP, DNA template including promoter
Transcribed Image Text:Mediator is not necessary to reproduce transcription initiation reactions in vitro. If you are assembling an in vitro system to study the formation of preinitiation complex in eukaryotes and the subsequent promoter scape, which of the following mixes will work? ο ο ο TFIIB-D-E-F- and H, NTPs, RNA polll, ATP, DNA template including promoter OTFIIB-D-E-F- and H, NTPs, RNA polll, DNA template including promoter TFIIB-D-E-F- and H, dNTPs, RNA polll, ATP, DNA template including promoter
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