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10. You have developed a transcription assay that recapitulates the in vivo regulation of the promoter for the RNA Pol II-transcribed Ratchet gene. By fractionating this extract, you have purified and cloned two specific transcription factors that, in addition to the general RNA Pol II transcription factors, are required for correct regulation of the promoter. As a first step to understand the function of these factors you make a series of deletion mutants of the Ratchet promoter and test their effect on transcription in the presence and absence of the Click and Clack factors. You find the following results. 3 5 7 Transcription +Click -Click -Clack +Clack + ++++ +++ +++ + +++ +++ +++ Based on this data, what would you predict the function of elements 3, 6, and 7 are?

10. You have developed a transcription assay that recapitulates the in vivo regulation of the promoter for the RNA Pol II-transcribed Ratchet gene. By fractionating this extract, you have purified and cloned two specific transcription factors that, in addition to the general RNA Pol II transcription factors, are required for correct regulation of the promoter. As a first step to understand the function of these factors you make a series of deletion mutants of the Ratchet promoter and test their effect on transcription in the presence and absence of the Click and Clack factors. You find the following results. 3 5 7 Transcription +Click -Click -Clack +Clack + ++++ +++ +++ + +++ +++ +++ Based on this data, what would you predict the function of elements 3, 6, and 7 are?

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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10. You have developed a transcription assay that recapitulates the in vivo regulation of the promoter for the RNA Pol II-transcribed Ratchet gene. By fractionating this extract, you have purified and cloned two specific transcription factors that, in addition to the general RNA Pol II transcription factors, are required for correct regulation of the promoter. As a first step to understand the function of these factors you make a series of deletion mutants of the Ratchet promoter and test their effect on transcription in the presence and absence of the Click and Clack factors. You find the following results. 3 4 ↓ 5 6 8 Transcription +Click +Clack -Click -Clack + +++ +++ + +++ +++ +++ Based on this data, what would you predict the function of elements 3, 6, and 7 are?
Transcribed Image Text:10. You have developed a transcription assay that recapitulates the in vivo regulation of the promoter for the RNA Pol II-transcribed Ratchet gene. By fractionating this extract, you have purified and cloned two specific transcription factors that, in addition to the general RNA Pol II transcription factors, are required for correct regulation of the promoter. As a first step to understand the function of these factors you make a series of deletion mutants of the Ratchet promoter and test their effect on transcription in the presence and absence of the Click and Clack factors. You find the following results. 3 4 ↓ 5 6 8 Transcription +Click +Clack -Click -Clack + +++ +++ + +++ +++ +++ Based on this data, what would you predict the function of elements 3, 6, and 7 are?
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