) By looking at the amino acid composition of the protein, we know it has 1 Tyr in it, but some how the professor had forgotten how many Trps where in the protein. Work out the number of Trps in the protein, given that the molar extinction coefficient at 280nm is 5500 M-1cm-1 for Trp and 1400 M-1cm-1 for Tyr. (b) If the absorbance at 280 nm of a solution of the above protein is 0.56, what is the concentration of the protein in solution, given that the pathlength of the cuvette is 1cm?
Enzyme kinetics
In biochemistry, enzymes are proteins that act as biological catalysts. Catalysis is the addition of a catalyst to a chemical reaction to speed up the pace of the reaction. Catalysis can be categorized as either homogeneous or heterogeneous, depending on whether the catalysts are distributed in the same phase as that of the reactants. Enzymes are an essential part of the cell because, without them, many organic processes would slow down and thus will affect the processes that are important for cell survival and sustenance.
Regulation of Enzymes
A substance that acts as a catalyst to regulate the reaction rate in the living organism's metabolic pathways without itself getting altered is an enzyme. Most of the biological reactions and metabolic pathways in the living systems are carried out by enzymes. They are specific for their works and work in particular conditions. It maintains the best possible rate of reaction in the most stable state. The enzymes have distinct properties as they can proceed with the reaction in any direction, their particular binding sites, pH specificity, temperature specificity required in very few amounts.
5. If we have a protein that has a molar extinction coefficient of 17900 M-1cm-1 at 280nm.
(a) By looking at the amino acid composition of the protein, we know it has 1 Tyr in it, but some how the professor had forgotten how many Trps where in the protein. Work out the number of Trps in the protein, given that the molar extinction coefficient at 280nm is 5500 M-1cm-1 for Trp and 1400 M-1cm-1 for Tyr.
(b) If the absorbance at 280 nm of a solution of the above protein is 0.56, what is the concentration of the protein in solution, given that the pathlength of the cuvette is 1cm?
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