2. Here is data to plot another STANDARD CURVE... Again, carefully consider how to scale and label the x and y-axes here. mm 5 10 20 40 80 kDa 200 100 50 25 10 Standard Use the resulting stander curve to estimate the size in kDa of a protein that travels 30 mm on a 12% polyacrylamide gel. (kDa)
2. Here is data to plot another STANDARD CURVE... Again, carefully consider how to scale and label the x and y-axes here. mm 5 10 20 40 80 kDa 200 100 50 25 10 Standard Use the resulting stander curve to estimate the size in kDa of a protein that travels 30 mm on a 12% polyacrylamide gel. (kDa)
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Polyacrylamide gel electrophoresis (PAGE) is a technique used to separate proteins based on their size and/or charge. It is commonly used to estimate the size of a protein and to confirm its purity.
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