2. Here is data to plot another STANDARD CURVE... Again, carefully consider how to scale and label the x and y-axes here. mm 5 10 20 40 80 kDa 200 100 50 25 10 Standard Use the resulting stander curve to estimate the size in kDa of a protein that travels 30 mm on a 12% polyacrylamide gel. (kDa)

2. Here is data to plot another STANDARD CURVE... Again, carefully consider how to scale and label the x and y-axes here. mm 5 10 20 40 80 kDa 200 100 50 25 10 Standard Use the resulting stander curve to estimate the size in kDa of a protein that travels 30 mm on a 12% polyacrylamide gel. (kDa)

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Question

2. Here is data to plot another STANDARD CURVE...
Again, carefully consider how to scale and label the x
and y-axes here.
mm
5
10
20
40
80
kDa
200
100
50
25
10
Standard
Use the resulting stemder curve to estimate the size in
kDa of a protein that travels 30 mm on a 12%
polyacrylamide gel.
(kDa)
February 14, 2023 6:10 PM
k Da

Expert Solution

Step 1

Polyacrylamide gel electrophoresis (PAGE) is a technique used to separate proteins based on their size and/or charge. It is commonly used to estimate the size of a protein and to confirm its purity.

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You ran a gel using SDS-PAGE. Total molecular mass of your unknown protein (determined via gel filtration): 217 kDa Standard curve equation based on your protein ladder: 5205.6x^(-1.22) Number of bands in your unknown protein sample: 3 Distance each band traveled on your gel: A measured at 20 mm B measured at 62 mm C measured at 82 mm What is the subunit composition of this protein? 2 of A, 1 of B, 1 of C 1 of A, 2 of B, 1 of C 1 of A, 1 of B, 2 of C 1 of A, 1 of B, 1 of C
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