a. Approximately how many amino acids are present in a 400 kDa protein. (show work) b. How many grams would there be in a nanomole of this protein? (show work) What is the purpose of adding DTT? d. How many subunits does the protein have, and what are their molecular weights?

Q: Which of the following might cause the denaturation of a protein? Select all that apply. A.)Highly…

A: Biological macromolecules are the molecules that are needed in a sufficient quantity for the…

Q: Distinguish the two methodologies used for getting a complete protein structure. Will the structural…

A: Proteins are essential for every cellular process occurring in a biological system as they control…

Q: Explain why unfolding or aggregation may occur using changes in entropy and enthalpy of the protein…

A: Adding a small amount of salt into a protein solution can increase the solubility of the protein in…

Q: The 3° structure of a protein refers to the protein's overall, 3-dimensional shape in space. This…

A: Amino acids are biomolecules that have an amino group, a carboxyl group and a side group that is…

Q: Some characteristics of three proteins are listed in the table below: Protein Molecular Weight…

A: Chromatography is a separation technique that separates a mixture of analytes based on their…

Q: Consider the peptides Pro-Gin-Val-Phe-His-Asp-Cys and His-Gln-Pro-Cys-Asp-Phe-Val. How do these two…

A: Amino acids are biomolecules in which an amino group and a carboxyl group are linked to the same…

Q: Match the following protein descriptions with the method that is best for determining the structure.…

A: The protein 3-D structure can be determined using techniques like NMR, X-ray crystallography, and…

Q: Describe the forces that are involved in the tertiary structure of a protein and give an example of…

A: A protein's biological function depends on its three-dimensional structure. The 3D structure is…

Q: Discuss in detail the protein denaturation, how denaturation occurs at levels of protein structure?

A: A macromolecule refers to the polymer that is composed of monomers, which are linked together by…

Q: You ran a gel using SDS-PAGE. Total molecular mass of your unknown protein (determined via gel…

A: To determine the molecular weight of subunit C from the SDS-PAGE data, we'll use the provided…

Q: Give typing answer with explanation and conclusion On paper draw a dipeptide, clearly showing the…

A: A dipeptide is a molecule consisting of two amino acids joined by a single peptide bond. The peptide…

Q: Consider a small protein containing 90 amino acid residues. The protein backbone will have 178 bonds…

A: Random coils are secondary structures that lack any regular pattern. Examples of secondary…

Q: Give two benefits of utilizing the biuret reaction to assess protein concentration over directly…

A: A Biuret test is a generic test performed by molecules having two or more peptide links (CO-NH…

Q: urrently, aspartic acid is forming an ionic interaction with arginine in a protein. Part a) If…

A: Proteins are the macromolecules composed of amino acids. The amino acids differ in their physical…

Q: a) Draw amino acid (L-Methionine) at low pH (pH= 1) b) Calculate the pl for amino acid Methionine

A: Isoelectric point is defined as the pH at which the amino acid exists as a zwitter ion. At this pH ,…

Q: Mr standards without with (kDa) 2-МЕ 2-МЕ 200 100 90 75 50 25 20 15 | | |I| | |||

A: Proteins are polypeptide molecules made up of amino acids. Proteins are synthesized through the…

Q: Protein A is made up of a single polypeptide chain; The molecular mass of A is 20,000 Da; the A…

A: Protein has various levels of the organization. The primary structure of protein consists only of…

Q: Physical methods are often used to determine protein conformation. Describe how x-ray…

A: Proteins- amino acids are the monomers for the production of proteins and contain nitrogen with…

Q: You are given a pure protein sample to characterize and provided the following information: Its…

A: Introduction  One or more long chains of amino acid residues make up the massive biomolecules and…

Q: You have a soluble protein that is highly flexible and is only 23 kDa in size. What is the most…

A: Proteins are the polymers of amino acids which are arranged in the three-dimensional structure of…

Q: You assay 0.5 ml of 1/2000 diluted egg white for protein, as described in the practical schedule,…

A: Standard calibration curves are plotted by taking concentration of the solution in x axis and…

Q: A mixture of three peptides is provided to you. The sequences of the three peptides are known: 1.…

A: Proteins are complex, high molecular weight biopolymers made up of 20 different amino acids linked…

Q: The A280 of a protein sample loaded onto a gel was determined to be 0.767 (1.00 cm path length,…

A: Measuring protein concentration directly from absorbance is a simple and faster way to quantify…

Q: A) List each of the five major functional classes of proteins. B) Discuss the function for each…

A: Amino acids are what makeup proteins. Protein structures are biopolymeric. Twenty amino acids are…

Q: Using one of the lower level structures, explain how the protein’s effects one of the higher level…

A: Proteins are biomolecules composed of amino acids. Unlike other biomolecules, proteins have…

Q: Using a detailed scheme, propose a step-wise protocol to purify protein B by ion exchange…

A: Given Values: PI value of protein A = 4.0 PI value of protein B = 6.5 PI value of protein C = 8.5 PI…

Q: Consider the following situation; Fresh pineapple contains the enzyme bromelain that hydrolyzes…

A: Fresh pineapple contains the enzyme bromelain that hydrolyzes peptide bonds in proteins.