a) The word “cloning” refers to making copies of bacteria making cloned sheep or another eukaryotic organism copying a piece of DNA all of the above none of the above b) Which of these restriction enzymes will create a sticky end? an enzyme that has restriction site ATTA and cuts between the two T nucleotides an enzyme with restriction site AGTCAG and cuts between the T and C an enzyme with restriction site AATCGG and cuts between the A nucleotides. any non-palindromic nucleotide sequence used as a restriction site none of the above c) Which of the following do researchers not need to use during vector cloning? a plasmid containing selectable marker genes such as beta galactosidase or ampicillin resistance genes restriction enzymes DNA polymerase a growth medium with carefully selected ingredients that take advantage of selectable markers. None of the above
Molecular Techniques
Molecular techniques are methods employed in molecular biology, genetics, biochemistry, and biophysics to manipulate and analyze nucleic acids (deoxyribonucleic acid (DNA) and ribonucleic acid (RNA)), protein, and lipids. Techniques in molecular biology are employed to investigate the molecular basis for biological activity. These techniques are used to analyze cellular properties, structures, and chemical reactions, with a focus on how certain molecules regulate cellular reactions and growth.
DNA Fingerprinting and Gel Electrophoresis
The genetic makeup of living organisms is shown by a technique known as DNA fingerprinting. The difference is the satellite region of DNA is shown by this process. Alex Jeffreys has invented the process of DNA fingerprinting in 1985. Any biological samples such as blood, hair, saliva, semen can be used for DNA fingerprinting. DNA fingerprinting is also known as DNA profiling or molecular fingerprinting.
Molecular Markers
A known DNA sequence or gene sequence is present on a chromosome, and it is associated with a specific trait or character. It is mainly used as a genetic marker of the molecular marker. The first genetic map was done in a fruit fly, using genes as the first marker. In two categories, molecular markers are classified, classical marker and a DNA marker. A molecular marker is also known as a genetic marker.
DNA Sequencing
The most important feature of DNA (deoxyribonucleic acid) molecules are nucleotide sequences and the identification of genes and their activities. This the reason why scientists have been working to determine the sequences of pieces of DNA covered under the genomic field. The primary objective of the Human Genome Project was to determine the nucleotide sequence of the entire human nuclear genome. DNA sequencing selectively eliminates the introns leading to only exome sequencing that allows proteins coding.
a) The word “cloning” refers to
- making copies of bacteria
- making cloned sheep or another eukaryotic organism
- copying a piece of DNA
- all of the above
- none of the above
b) Which of these restriction enzymes will create a sticky end?
- an enzyme that has restriction site ATTA and cuts between the two T nucleotides
- an enzyme with restriction site AGTCAG and cuts between the T and C
- an enzyme with restriction site AATCGG and cuts between the A nucleotides.
- any non-palindromic
nucleotide sequence used as a restriction site - none of the above
c) Which of the following do researchers not need to use during vector cloning?
- a plasmid containing selectable marker genes such as beta galactosidase or ampicillin resistance genes
- restriction enzymes
- DNA polymerase
- a growth medium with carefully selected ingredients that take advantage of selectable markers.
- None of the above
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