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In what procedure(s) might the degenerate base code be important?
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- What would be the modifier to add to this HCPCS code?What is experiment of Thermodynamics of Small Oligomeric Duplex DNA Denaturation.If the code were a sextuplet (containing of six nucleotides), would the reading frame be restored by the addition or deletion of three, six, or nine nucleotides? Would the reading frame be restored by the addition or deletion of three, six, or nine nucleotides?
- During agarose gel electrophoresis, why does DNA move through the gel when electric current is applied? because DNA is negatively charged because a charged chemical from the loading buffer is bound to the DNA because DNA is positively charged because DNA absorbs electricityIn Polymerase Chain Reaction (PCR), the temperature is one of the most important parameters that could influence the efficiency of this technique. Each cycle of this reaction has its own specific temperature. For instance, the denaturation step possesses a temperature of 94 - 98 ℃ to ensure that the double stranded DNA is fully separated. (i) (ii) (iii) Why is the annealing temperature vital in this technique? Explain how will this temperature affects the efficiency of this reaction. Why is Hot Start PCR technique preferred by some researchers? If the primers you purchased possessed the following information. 5'-GGA AAC AGC TAT GAC CAT G-3' Calculate the melting temperature of this primer and estimate the annealing temperature of this primer.Why do DNA chips often contain segments derived from cDNA rather than genomic DNA segments?
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