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- question 2QUESTION 3 You obtained the following raw data when setting up a Bradford standard curve: BSA (mg/ml) Absorbancy 595nm 0.225 1 0.310 0.420 3 0.510 4 0.610 0.720 0.810 7 0.915 8 0.950 9 0.980 10 0.990 Set up a standard curve. What would the quality of the line-fit (R2 value) be if you exclude experimental outliers? (Give you answer to 4 decimal places)Question 4: Starting with a culture that contains 5.0 x 107 CFU/ml, describe a serial dilution scheme that would give a plate with 50 colonies.
- QUESTION 4 You obtained the following raw data when setting up a Biuret standard curve: BSA (mg/ml) 0 1 2 3 4 5 6 7 8 9 10 Absorbancy 540nm 0.158 0.210 0.260 0.305 0.360 0.410 0.455 0.510 0.530 0.550 0.554 What would the quality of the line-fit (R2 value) be if you do not exclude experimental outliers? (Give you answer to 4 decimal places)Question 7: A. Calculate how to make 10 ml of a 7.5% methylene blue stock solution (from solid powder). B. Calculate how to make a series of 1:10 dilutions of this stock. Imagine you'll use 5 ml of each dilution for your experiment. Calculate the final concentrations of each tube. tube 1: take some stock, dilute it 1:10 with some water in a new tube - #1 tube 2: take some of #1, dilute it 1:10 with some water in a new tube tube 3: take some of #2, dilute it 1:10 with some water in a new tube tube 4: take some of #3, dilute it 1:10 with some water in a new tube C. First, decide the total volume to make of each tube-keep in mind measurement accuracy (consider preparing 20% more solution than what you will use). D. Then, calculate how much stock, water and final concentration of each. E. Then, instead of doing a serial dilution, calculate making each of the above solutions using the weight/volume % solution method. F. Compare and contrast serial dilution and W/V % methodsQuestion 3. Your professor asks you to make a staining solution to stain and observe specimens under the microscope. You are told to prepare 100 mL of staining solution containing 40% methanol, 1X hematoxylin dye and 0.01 M calcium chloride (CaC12). In the histology lab, you find a stock of 100% pure methanol (liquid), a 10x hematoxylin dye stock solution (liquid), plenty of water and a stock solution of 5M CaC12. There are also all kinds of pipets, cylinders and beakers to measure volumes. a) Describe how you make the staining solution? b) On your following trip to the histology lab, you realize that all the pipets to accurately measure volumes smaller than 1 ml are broken. What alternative method do you need to use to avoid pipetting small volumes (just explain in a couple of sentences, no calculation needed)?
- Question 1. You are given a P1000 & a P5000 micropipette to validate by measuring the weight of distilled water at the maximum volume and minimum volume for both pipettes. Each volume is tested five times and you recorded the weight as given by the balance (recorded in grams). Results P1000 Weight 1 (g) Weight 2 (g) | Weight 3 (g) | Weight 4 (g) Weight 5 (g) 1000 µl 1.1 0.95 0.94 0.98 0.97 100 µl 0.10 0.14 0.11 0.10 0.11 Does this micropipette pass validation? Show your workings and comment on how you reached your decision. P5000 Weight 1 (g) | Weight 2 (g) Weight 3 (g) Weight 4 (g) Weight 5 (g) 5000 µl 5.30 5.32 5.25 5.17 5.20 1000 µl 0.75 0.84 0.81 0.77 0.79 Does this micropipette pass validation? Show your workings and comment on how you reached your decisionQUESTION 4 You obtained the following raw data when setting up a Bradford standard curve: BSA (mg/ml) Absorbancy 595nm 0.225 1 0.310 0.420 0.510 4 0.610 0.720 6 0.810 7 0.915 8 0.950 9. 0.980 10 0.990 Set up a standard curve. What would the quality of the line-fit (R2 value) be if you do not exclude experimental outliers? (Give you answer to 4 decimal places) N MQuestion 5 We can determine the copper(II) ion content of a sample using spectrophotometry. The following data was collected. Construct a calibration plot (x - [Cu2+], Y- Absorbance)and determine the slope. Absorbance [Cu2+], M 0.065 2.00E-06 0.205 6.00E-06 0.338 1.00E-05 0.474 1.40E-05 0.598 1.80E-05 3.3 x 10-2 3.3 x 104 3.1 x 10-5 2.3 x 10-3
- Question 5 a) Explain the general diagnostic principles using radiopharmaceuticals by which medical images may obtained. b) Describe the use of technetium-99m-MDP. c) Describe the use of 1251 radioisotope. [Suggested word count 270] [2.2]Answer question number 2QUESTION 1 What is 105 ng ml in: a. ug/ml b. mg 1 C. ug1 d. g/ul (Please list each answer on a separate line, beginning with the corresponding letter. Don't forget units for each answer!) For the toolbar, press ALT+F10 (PC) or ALT-FN+F10 (Mac). 14px E E YA BIUS Paragraph Arial 田 Bヨ田田用 国 田EEt o