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1. The primers used in colony PCR will target EGFP genes. Write down your expected results for each case. You should present the amplicon size(s) in your answer. 1) No ligation 2) Self-ligation of two vectors 3) Self-ligation of two inserts 4) Ligation of a vector with multiple inserts. Also, how many inserts can be ligated into a vector? 5) Ligation of one insert into a vector

1. The primers used in colony PCR will target EGFP genes. Write down your expected results for each case. You should present the amplicon size(s) in your answer. 1) No ligation 2) Self-ligation of two vectors 3) Self-ligation of two inserts 4) Ligation of a vector with multiple inserts. Also, how many inserts can be ligated into a vector? 5) Ligation of one insert into a vector

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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The primers used in colony PCR will target EGFP genes. Write down your expected results for each case. You should. present the amplicon size in your answer. 

1) ligation

2) self ligation of two vectors

3) self ligation of two inserts

4) ligation of a vector with multiple inserts. also how many inserts can be ligated into a vector

5) ligation of one insert into a vector 

1. The primers used in colony PCR will target EGFP genes. Write down your expected results for each case. You should present the amplicon size(s) in your answer. 1) No ligation 2) Self-ligation of two vectors 3) Self-ligation of two inserts 4) Ligation of a vector with multiple inserts. Also, how many inserts can be ligated into a vector? 5) Ligation of one insert into a vector
Transcribed Image Text:1. The primers used in colony PCR will target EGFP genes. Write down your expected results for each case. You should present the amplicon size(s) in your answer. 1) No ligation 2) Self-ligation of two vectors 3) Self-ligation of two inserts 4) Ligation of a vector with multiple inserts. Also, how many inserts can be ligated into a vector? 5) Ligation of one insert into a vector
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