Day 4 - 7: Blastocyst Stage Draw and label: Trophoblast, blastocoel, and inner cell mass. Indicate the potency (fully differentiated, multipotent, pluripotent, or totipotent) of the cell(s) at this stage. Day 8-12: Blastocyst Stage Draw and label: epiblast and hypoblast. The epiblast develops into the The hypoblast develops into the Day 13 - 15: Gastrulation Fill in the blanks below and label the image. At this stage, the cells of the primitive ectoderm begin to migrate and form a structure called the (light arrow). The invaginating cells then develop into the and the

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Arrange the cytoplasmic rearrangement of the fertilized egg of the tunicate, Styela partita in consecutive order. (I) After sperm entry into the vegetal hemisphere of the oocyte, the yellow cortical cytoplasm and the clear cytoplasm derived from the breakdown of the oocyte nucleus contract vegetally toward the sperm.(II) Before fertilization, yellow cortical cytoplasm surrounds the gray (yolky) inner cytoplasm. (III) The final position of the yellow cytoplasm marks the location where cells give rise to tail muscles.(IV) As the sperm pronucleus migrates animally toward the newly formed egg pronucleus, the yellow and clear cytoplasm move with it.

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