A PCR-RFLP analysis was performed to identify PTC tasters in a population. The taster allele sequence included a palindromic sequence recognized by a restriction enzyme, whereas the non-taster allele did not due to a SNP in the sequence. The gel shows the results of the analysis from 100 individuals. The numbers above the gel lanes represent the number of individuals with each genotype. 33 52 15 Based on the results from the gel, what is the frequency of the taster allele in this population? 0.0387 0.41 0.33 0.685 0.59 0.52 0.315 0.0574 Screenshot

Q: an insert check (labeled A-D below). After growing cultures, you isolate the plasmids and treat them…

A: In E. Coli lac operon contains a structural gene lac Z that codes for the enzyme beta galactosidase.…

Q: In Ex 1.1 we used the Wizard DNA isolation kit to isolate the pCR2.1 vector and in Ex 1.11 we used…

A: The Wizard DNA isolation Kit is designed for the isolation of DNA from tissue culture cells, animal…

Q: How many fragments would be produced if the DNA is cut by that enzyme?    Number each fragment…

A: * Restriction enzymes recognizes specific palindrome sequence and cut DNA only at that specific…

Q: Lane assignments: 1:1 kb MW Ladder 2-3: Pair 1 4-5: Pair 2 6-7: Pair 3 8: PCR Product 9: Negative…

A: In this inquiry, we investigate how to get it a PCR experiment-related agarose gel electrophoresis…

Q: Comment on the failure of p1vir transduction experiments and give a reason why you think the…

A: transduction experiments is a technique commonly used in molecular biology to introduce foreign DNA…

Q: You have a DNA sequence of 1800 bp. To characterize it, you decide to make a restriction map.…

A: Restriction mapping is a process that helps to determine the location of the restriction site. The…

Q: The figure below represents the size of various SSRS that are used for forensic analysis. The bars…

A: vWA produce 200bp in PCR . All others produce less or more than 200 bp in PCR.  Here SSR means is…

Q: The synthesis of phosphocreatine is unfavorable. If dur- ng extended periods of rest, e.g., sleeping…

A: Phosphocreatine (PCr) -  It is a phosphorylated creatine molecule that works as transferable reserve…

Q: Explain the principles of DNA migration of sample A in the electrophoresis experiment

A: Electrophoresis is a separation technique that was used to separate the mixture of molecules from a…

Q: c) Explain the centrifugation pattern, with reference to heavy (N5) and light (N) nitrogen-…

A: DNA contains a significant amount of nitrogen. Although 14N is the most prevalent nitrogen isotope,…

Q: You would like to amplify the gene shown here using PCR.  Assume the primer to the 5' end of the…

A: PCR stands for Polymerase Chain Reaction. This is used to amplify the gene of interest.

Q: The following figure shows the FBI-style analysis of the genomic DNA of 10 people (1-10), and also…

A: Chromosomes are carrier of deoxyribonucleic acid (DNA). DNA is the genetic material. Simple sequence…

Q: The short sequence shown below is part of a gene of interest. You decide to amplify this part of the…

A: In PCR, primers serve as the initial building block for DNA synthesis. DNA synthesis is carried out…

Q: What are some of the observations and conclusions that can be made from gel bands for 16s PCR

A: Answer :- Here are some observations :- 1) Increase hardening temperature by increments of two…

Q: For a molecular cloning experiment using the bacterial strain E. coli K12 is used to insert the gene…

A: Recombinant DNA technology uses enzymes and a variety of laboratory procedures to modify and isolate…

Q: After four cycles of PCR, which products predominate? Explain why.

A: Polymerase chain reaction (PCR) is a type of genetic analysis in which the nucleotide sequence of…

Q: You work in an environmental microbiology research lab, and your project is to design a recombinant…

A: Our task as research assistants in an environmental microbiology lab is to create a recombinant…

Q: Select the definition of a microsatellite. short pieces of DNA surrounding the periphery of the…

A: Answer

Q: Lane 1 = TakaraBio 200 bp DNA ladder Lane 2 = Haell only; (thick band) 1883 bp and 1871 bp, 799 bp…

A: Understanding the restriction digestion patterns of any plasmid is very important to know where…

Q: Researchers are manipulating the gene cxx2 for their experiments, and they have inserted a small…

A: As per the central dogma of biology, the flow of genetic information is from DNA -> RNA ->…

Q: A fellow lab worker brings you DNA containing what might be a similar gene in Leopard Geckos (XG).…

A: Introduction: The polymerase chain reaction is an innovative method for rapid amplifying specific…

Q: Describe what occurs in each of the three steps during the PCR reaction.

A: POLYMERASE CHAIN REACTION It is a method to rapidly make millions to billions of copies of a…

Q: Questions 11-14. Melia and Sophie performed a PCR experiment using DNA purified from their phage…

A: * PCR is the polymerase chain reaction is a molecular techniques that forms invitro conditions. * It…

Q: [GAPDH] is varied per sample. Cannot be determined [GAPDH] is comparable per sample. [GAPDH] is not…

A: PCR :It stands for polymerase chain reaction.It is a rapid and versatile in vitro method for…

Q: Here you see the gel electrophoresis of PCR reactions of the pMCT118 VNTR locus of 4 individuals…

A: Answer pMCT118 VNTR  locus has multiple repeats that can be analyzed by Polymerase chain reaction…

Q: parental and recombinant offsprings

A: Parental offsprings: These are the offsprings that have the same combination of alleles as one of…

Q: Which of the following is a correct statement about the primers used in the ALU insertion PCR…

A: Answer -  Option B - The primers for both + and - alleles are the same and are on flanking regions…

Q: Differentiate between TWO of the following pairs: Genetic and a restriction map Southern and…

A: The mapping of DNA refers to the identification of the location of particular genes. Genetic map and…

Q: You performed RADseq analysis of two genomes using the restriction enzyme EcoRI. From each genome,…

A: * Restriction site associated DNA (RAD) markers are genetic marker that are used for association…

Q: When different individuals within a population have different numbers of restriction sites within…

A: Introduction: A restriction site in DNA is a group of 6–8 base pairs that a restriction enzyme…

Q: In your PCR reaction, you were able to use the same primer set, even though you were likely…

A: PCR is a molecular biology tool that is useful in a variety of ways. It is used in forensic…

Q: Which female could be the mother of the child and why? Which male could be the father of the child…

A: There are several ways to identify the mother of a child from VNTR loci. One way is to look at the…

Q: Someone broke into the bookstore and stole thousands of dollars’ worth of textbooks. Some hairs were…

A: Most likely the culprit is suspect 1. This is so as the banding pattern of suspect 1 is matching…

Q: Are any of the loci on the X chromosome? If so, identify this (these) locus (loci) by color.

A: The term SSR stands for simple sequence repeat that is a microsatellite VNTR (variable number tandem…

Q: You would like to amplify the gene shown below using PCR with two primers: the primers are shown on…

A: Explanation: A specific area of DNA is amplified using PCR. Primers, which are compact fragments of…

Q: genetic disease is caused by a deletion in part of a gene. The deletion results in one copy of the…

A: Option b

Q: What is the formula used to calculate the number of DNA molecules which will be created for a given…

A: PCR or polymerase chain reaction is a technique of DNA amplification. Here a small amount of DNA is…

Q: The following DNA sequence has been identified and named ‘Gene Z’. It is thought that this sequence…

A: PCR is a polymerase chain reaction, this is a biotechnological process which is used to amplify the…

Q: Primers can sometimes bind and target the wrong gene, especially if the primers are allowed to bind…

A: It is difficult for one to to find out gene of interest out of a complete genome. However, a given…

Q: You want to amplify a specific region of DNA during PCR. Your primers should

A: Primeras are short sequences of DNA with up to 18-20 base pairs composed of nitrogenous bases (…

Q: The human gene for hemophilia is on the X chromosome. Below is a pedigree from a family afflicted…

A: Hi, Thanks For Your Question.  Answer : Correct Option Is b (20%) Explanation :  P (III-7 Is…

Q: You are studying a genetic disease and trying to determine its location on a chromosome using…

A: Gel electrophoresis is used to separate the DNA fragments on the basis of their size.

Q: You are considering using restriction enzymes that recognizes 4-bp and 6-bp nucleotide sequences,…

A: From theory we know, the longer the restriction site recognized by a particular restriction…

Q: When demonstrating horizontal gene transfer, why is it important to use strains that each require at…

A: Introduction  Gene transfer is referred to the process in which the DNA (Deoxyribose Nucleic Acid)…

Q: Below is the DNA sequence (coding strand) for the human placental protein 14 (not all of the gene…

A: PCR stands for Polymerase Chain Reaction. It's a widely used laboratory technique in molecular…

Q: n lane 1, a size standard was loaded, which contained a mix a DNA fragments known to be 1000 bp, 700…

A: Electrophoresis is a method used to separate DNA, RNA, and protein molecules which depends on their…

Microbial Taxonomy

Taxonomy is the science of naming, classifying, and describing organisms. The taxonomy will include all the plants, microorganisms, and animals on this planet. Carl Linnaeus coined the taxonomy term. He is known as the father of taxonomy. Microbial taxonomy is a criterion of grouping microorganisms into groups based on their similarities and past relatedness. Microorganisms belonging to the same group are differentiated from the others via grouping into categories. This differentiation makes the study of microorganisms easy. There are different types of microbial taxonomies like Numerical and Molecular taxonomy. The study of microorganisms is known as microbiology. Microbiology is a broad term that takes into account microbial cultures, taxonomic studies, evolutionary relationships. Microbial interaction and diversity are huge data that needs to be compiled under the subject of microbiology. Microbiology covers various organisms like bacteria, fungi, algae, plants, animals, etc.