fellow lab worker brings you DNA containing what might be a similar gene in Leopard Geckos (XG). She asks you to see if you can amplify it using the same primers you used in frogs and the Bearded Dragon. You run the PCR and then analyze the product by running the DNA on an agarose gel. As a control, you run out the PCR product you amplified in Bearded Dragons and a DNA “ladder” of DNA pieces of known sizes. The gel results are shown below. Marker = DNA ladder; Lane A = Bearded Dragon PCR product; Lane B = Leopard Gecko PCR results. In the box below, determine the sizes of the PCR products shown in Lanes A & B.Size of Bearded Dragon PCR product: ______________________________ Sizes of Leopard Gecko PCR products: ______________________yes or No - based on the results shown in the gel above – would you say the frog PCR primers for gene X amplified the same gene in the Bearded Dragon? The image is a representation of an agarose gel electrophoresis result, commonly used in molecular biology to separate DNA fragments by size. Here's a detailed description for educational purposes:### Gel Diagram Explanation**Lanes:**1. **Marker Lane:** - This lane contains a DNA ladder with fragments of known sizes. It serves as a reference to estimate the size of DNA fragments in the sample lanes. - The sizes of the marker fragments are labeled on the left in base pairs (bp), ranging from 100 bp to 1,200 bp.2. **Sample Lanes:** - **Sample A:** Shows a prominent band around 900 bp. - **Sample B:** Displays a band approximately at 1,000 bp. - **Sample C:** Features two bands, one near 700 bp and another at 200 bp.### Interpretation:- **Marker Lane:** Provides a scale for determining the size of DNA fragments in other lanes.- **Sample A:** Contains DNA mostly around 900 bp.- **Sample B:** Contains DNA predominantly around 1,000 bp.- **Sample C:** Contains two distinct DNA fragments at approximately 700 bp and 200 bp.This image helps in understanding how DNA fragments can be visualized and sized using gel electrophoresis, a fundamental technique in genetic analysis and research.

Answered: fellow lab worker brings you DNA…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Question

fellow lab worker brings you DNA containing what might be a similar gene in Leopard Geckos (X_G). She asks you to see if you can amplify it using the same primers you used in frogs and the Bearded Dragon. You run the PCR and then analyze the product by running the DNA on an agarose gel. As a control, you run out the PCR product you amplified in Bearded Dragons and a DNA “ladder” of DNA pieces of known sizes. The gel results are shown below. Marker = DNA ladder; Lane A = Bearded Dragon PCR product; Lane B = Leopard Gecko PCR results. In the box below, determine the sizes of the PCR products shown in Lanes A & B.

Size of Bearded Dragon PCR product: ______________________________

Sizes of Leopard Gecko PCR products: ______________________

yes or No - based on the results shown in the gel above – would you say the frog PCR primers for gene X amplified the same gene in the Bearded Dragon?

The image is a representation of an agarose gel electrophoresis result, commonly used in molecular biology to separate DNA fragments by size. Here's a detailed description for educational purposes:

### Gel Diagram Explanation

**Lanes:**

1. **Marker Lane:**
- This lane contains a DNA ladder with fragments of known sizes. It serves as a reference to estimate the size of DNA fragments in the sample lanes.
- The sizes of the marker fragments are labeled on the left in base pairs (bp), ranging from 100 bp to 1,200 bp.

2. **Sample Lanes:**
- **Sample A:** Shows a prominent band around 900 bp.
- **Sample B:** Displays a band approximately at 1,000 bp.
- **Sample C:** Features two bands, one near 700 bp and another at 200 bp.

### Interpretation:

- **Marker Lane:** Provides a scale for determining the size of DNA fragments in other lanes.
- **Sample A:** Contains DNA mostly around 900 bp.
- **Sample B:** Contains DNA predominantly around 1,000 bp.
- **Sample C:** Contains two distinct DNA fragments at approximately 700 bp and 200 bp.

This image helps in understanding how DNA fragments can be visualized and sized using gel electrophoresis, a fundamental technique in genetic analysis and research.

Expert Solution

Step 1

Polymerase chain reaction (PCR) involves amplification of a nucleotide sequence using respective primer set and highly thermostable DNA polymerase (Taq polymerase). The DNA amplified can be run on an agarose gel to get an idea of fragment size that has been amplified during the reaction.