9) Below, there is a representation of an SDS-PAGE gel. Assuming the samples in the MW standard have masses of: 66 kDa, 45 kDa, 36 kDa, 29 kDa, 24 kDa, 20.1 kDa, and 14.2 kDa, a) Figure 4: indicate where each of the measurements were taken and label as in II.6. figure 2 above. b) As in II.7. Table 1 above create Table 2 using the data below. Determine the r.f. values for the MW standards, plot the relative mobility versus the log of the mass for the standards, and use the best fit straight line to determine the molecular weights of the proteins in the whey, peak 1, and peak 2 lanes. (5 pts—this will be scaled up appropriately if your gel did not develop properly) dye MW Whey Peak 1 Peak 2
9) Below, there is a representation of an SDS-PAGE gel. Assuming the samples in the MW standard have masses of: 66 kDa, 45 kDa, 36 kDa, 29 kDa, 24 kDa, 20.1 kDa, and 14.2 kDa, a) Figure 4: indicate where each of the measurements were taken and label as in II.6. figure 2 above. b) As in II.7. Table 1 above create Table 2 using the data below. Determine the r.f. values for the MW standards, plot the relative mobility versus the log of the mass for the standards, and use the best fit straight line to determine the molecular weights of the proteins in the whey, peak 1, and peak 2 lanes. (5 pts—this will be scaled up appropriately if your gel did not develop properly) dye MW Whey Peak 1 Peak 2
Chapter1: Relative Value, Addition, And Subtraction Of Decimals
Section: Chapter Questions
Problem 39SST
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Transcribed Image Text:9) Below, there is a representation of an SDS-PAGE gel. Assuming the samples in the
MW standard have masses of: 66 kDa, 45 kDa, 36 kDa, 29 kDa, 24 kDa, 20.1 kDa, and
14.2 kDa,
a) Figure 4: indicate where each of the measurements were taken and label as in II.6.
figure 2 above.
b) As in II.7. Table 1 above create Table 2 using the data below. Determine the r.f.
values for the MW standards, plot the relative mobility versus the log of the mass
for the standards, and use the best fit straight line to determine the molecular
weights of the proteins in the whey, peak 1, and peak 2 lanes. (5 pts—this will be
scaled up appropriately if your gel did not develop properly)
dye
MW
Whey
Peak 1 Peak 2
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