If you had added 1.0 mL of 1.0 M HCl to your 50 mM potassium phosphate buffer solution, would it still have been a valid buffer? Include your calculations to support your answer.
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- If you had added 1.0 mL of 1.0 M HCl to your 50 mM potassium phosphate buffer solution, would it still have been a valid buffer? Include your calculations to support your answer.
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- Determine the molarity of 3.72 moles of NaBr in 575mL of solution. (MM NaBr= 102.89 g/mol) Report your answer to 3 significant figures. Do not include the unit when writing your answer.You have a 100X stock of a buffer. You need 100 ml of 1X buffer. How much buffer solution do you use? How much water?How do you prepare a 100mL of 0.1 M phosphate buffer? From the anterior buffer, how do you make 100mL of 0.05 M? PLEASE GIVE THE MEASUREMENTS
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- Using your data, explain your observations for the pH changes upon the addition of acid/base to your 50 mM buffer, the 5 mM buffer, and the distilled water. Complete the calculations below. Use your notebook for scratch paper if needed. For each solution, you will need to calculate the total moles of buffer (if applicable), the moles of monobasic, the moles of dibasic, and the moles for each addition of acid/base. Determine the expected pH for the first addition of acid/base, the second addition of acid/base and compare to your measured values. 50 mM Buffer Calculations A- moles HA moles Moles total = 2.5 x 10^-3 1.45 x10 ^-3 1.5 x10 ^-3 Addition of Acid/Base 1st addition moles added = 2nd addition moles added = 5mM Buffer Calculations A- moles HA moles Moles total = Addition of Acid/Base 1st addition…Using your data, explain your observations for the pH changes upon the addition of acid/base to your 50 mM buffer, the 5 mM buffer, and the distilled water. Complete the calculations below. Use your notebook for scratch paper if needed. For each solution, you will need to calculate the total moles of buffer (if applicable), the moles of monobasic, the moles of dibasic, and the moles for each addition of acid/base. Determine the expected pH for the first addition of acid/base, the second addition of acid/base and compare to your measured values. 50 mM Buffer Calculations A- moles HA moles Moles total = 2.5 x 10^-3 1.45 x10 ^-3 1.5 x10 ^-3 Addition of Acid/Base 1st addition moles added = 2nd addition moles added = 5mM Buffer Calculations A- moles HA moles Moles total = Addition of Acid/Base 1st addition…You need to prepare 1L of a 5×TE stock solution (1×TE buffer:10mM Tris,1mM EDTA).How much of Tris you need? How many ml of the 0.5 EDTA stock solution do you need?