You have a 12 mL sample of acetylcholine( a neurotransmitter) with an unknown concentration and a pH of 7.86. You incubate this sample with the enzyme acetylcholinesterase to convert all of the acetylcholine to choline and acetic acid. The acetic acid dissociates to yield acetate and hydrogen ions. At the end of the incubation period, you measure the pH again and find that it has decreased to 5.68. Assuming there was no buffer in the assay mixture, determine the number of nanomoles of acetylcholine in the original 12 mL sample.

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