1. DNA can be cut at specific sequence motifs using enzymes called restrictionendonucleases. You obtain a single PCR product from R. sphaeroides DNA that can be cut inthe places shown below by solid arrows. These are the Clal specific restriction motifs.Positions of these restriction sites are indicated by nucleotide numbers in a 1059 nucleotideDNA fragment. Draw the expected band(s) for each of the bacterial species on the gelpicture below if treated with the restriction enzymes as illustrated.Rhodobacter sphaeroides 2. 4.110590290749Rhodopseudomonas palustris BisB50Dinoroseobacter shibae DFL 120290Silicibacter sp. TM 10400105974910591059DNA ladderR. sphaeroides 2. 4. 1D. shibae DFL12R. palustris BisB5Silicibacter sp. TM104011001000900800700600500400300200100||||||||CS Scanned with CamScanner54

Solution:Digestion of DNA with ClaI restriction enzyme will produce restriction fragments as given…

Answered: 1. DNA can be cut at specific sequence…

Biology Today and Tomorrow without Physiology (MindTap Course List)

5th Edition

ISBN:9781305117396

Author:Cecie Starr, Christine Evers, Lisa Starr

Publisher:Cecie Starr, Christine Evers, Lisa Starr

Chapter10: Biotechnology

Section: Chapter Questions

Problem 8SQ

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The map of plasmid pUC19 is shown below. The restriction site coordinate is the position of the 5’base on the top strand of each site sequence. The restriction enzyme sites are in bold type if there is only one site in pUC19. Please list the fragments in order of size, largest to smallest, which will result from a complete digestion by the restriction enzyme PvuII. Please list the fragments in order of size, largest to smallest, which will result from a complete digestion by the restriction enzyme DrdI.
Kha Vu Danels Include: 8DX : Safehy Jor bromie tnto lab repart! Name Section date sheet MAPPING PRACTICE #1 Below is a restriction map for the plasmid PGEN 101 (total length = 20 Kb). Using this map as a guide, give the number of restriction fraqments along with their associated lengths that would result from digesting PGEN 101 with the restriction enzymes EcoRI, BamHI and a combination of ECORI and BamHI. BamHI 3.2 Kb 1.7 Kb EcoRI BamHI PGEN 101 8.7 Kb 5.5 Kb .9 Kb EcoRI ECORI DIGESTION PERFORMED SIZES OF FRAGMENTS OBTAINED 10.4 kb , 0.9kb, 8.7 Kb EcoRI 3.2 Kb, 16. 8kb BamHI EcoRI + BamHI
This is a restriction map for the 250 base pair plasmid pSage. Restriction sites for the restriction endonuclease Nhel are 7, 69 and 160. What are the sizes of the restriction fragments produced? Check all that apply. p SAGE Nhel 7 250 bp Nhền 160 Nhel 69 62 69 91 160 97
Assume that a plasmid is 4700 base pairs in length and has restriction sites for a given restriction enzyme at the following locations: 800, 1400, 2900, and 3600. List the fragments by size that are ! expected when the plasmid is fully digested the restriction enzyme.
Below is a plasmid with restriction sites for Baml and EcoRI, Several restriction digests were done using these two enzymes either alone or in combination. Hint: Begin by determining the number and size of the fragments produced with each enzyme. "kb" stands for kilobases, or thousands of base pairs. Plasmid Gel lanes I | III IV V 6 Kb Bam HI Bam HI. 20 Kb PGEN101 (20 Kb) 2 Kb 11 Kb Bam HI 8 Kb 8 Kb 4 Kb 6 Kb Eco RI 3 Kb 21. Which lane shows a digest with BamHl only? a. I b.I c. II d. IV e. V 22. Which lane shows a digest with EcoRLonly? a. I 23. Which lane shows the fragments produced when the plasmid was incubated with both EcoRI and BamH1? a. I b.I c. II d. IV e. V b. I c. II d. IV e. V Base pairs
1. Briefly explain why the total size of the pMBBS plasmid in the Restriction Enzymes practical is 3000bp (base pairs) 2. Briefly explain why the cut sites on the pMBBS plasmid in each Restriction Enzymes (EcoRI, BamHI, and XhoI) just 1 each 3. Briefly explain what led to the 5 fragments formed which linked to the 500bp, 1000bp, 1500bp, 2000bp, 2500bp sizes
1. A plasmid was digested with two restriction enzymes, individually and in a double digest. The following fragment sizes (bp) were determined by agarose gel electrophoresis. Restriction Enzyme|Fragment Size (bp) 30, 15, 5 HindIII 50 EcoRI + HindIII 20, 15, 10, 5 Make a restriction map of the plasmid based on this data and indicate the distances between the restriction sites.
= Menu #1 Mol Bio Restriction An... X + Create All tools Edit Convert E-Sign Sign in Find text or tools H 3. If the PMBBS plasmid were digested with all three REs together, how many fragments would be observed, and what would be the sizes (in bp) of these fragments? Number of fragments: Sizes: 4. Draw the actual map of the PMBBS plasmid, following the style of the sample map shown in Figure 1. Make sure you show the following information: (1) relative locations of the RE sites; (2) size in base pairs of the segments of DNA between sites. (The map need not be drawn to scale.) Answer: (provide a drawing) NFL Q Search IA W ☑ 0 Al Assistant C 2 2 C 1:1 ༢ ☑ 8:58 PM 10/22/2024 Q
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9. Here is a restriction map for a bacterial plasmid showing the cleavage sites for two different restriction enzymes EcoRI and Bam H1. EcoR1 700 bp EcoR1 2000 bp 1200 bp BamH1 Match the banding pattern in each lane of the agarose gel below with one of the treatments listed. Note: Not all treatments listed are run on the gel. Plasmid treatments: A. BamHI+EcoR1 (no RNAse) B. BamHI only (no RNAse) Gel lanes: 1. DNA Ladder = Mol. Wt. Standard (sizes indicated) bp 5000 4000 3000 2000 1650 1000 850 650 500 C. BamH1 + EcoR1 + RNAse D. RNAse only 2. E. BamH1 + RNAse F. EcoR1+ RNAse 3. G. EcoR1 only (no RNase) 4. 1 2 3 4 5 6 5. 6. -
5. You wish to map restriction sites in an unknown plasmid that has not been sequenced. You digest the plasmid with combinations of EcoRI, SalI, and BamHI, producing fragment sizes as indicated in the table below. Draw a restriction map of the plasmid, indicating relative positions and distances between restriction sites. Restriction Enzyme(s) used | Resulting Fragment Sizes EcoRI 5386 Sall 5386 1079, 4307 3078, 2308 331, 1079, 3976 898, 1079, 3409 ВатHI EcoRI + Sall ЕcoRI + BamНІ Sall + BamHI
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