1 1. The structure of a human gene is given below, with boxes indicating exons and lines indicating introns. Assume complete splicing. Arow indicate position and direction (5'→ 3') of primers used for PCR. Sizes of each segment in kb are indicated below the diagram. Draw the expected results on the gel below for each experiment. Lane A: PCR of genomic DNA using primers 3 and 4. Lane B: PCR of cDNA using primers 3 and 4 Lane C: PCR of cDNA using primers 2 and 4 Lane D: PCR of cDNA using primers 1 and 4 2 3 2 kb 0.5 kb 2 kb 0.5 kb 1 kb 2.5 kb 1 kb 10 9 8 7 6 5 4 3 2 A B C D 4

1 1. The structure of a human gene is given below, with boxes indicating exons and lines indicating introns. Assume complete splicing. Arow indicate position and direction (5'→ 3') of primers used for PCR. Sizes of each segment in kb are indicated below the diagram. Draw the expected results on the gel below for each experiment. Lane A: PCR of genomic DNA using primers 3 and 4. Lane B: PCR of cDNA using primers 3 and 4 Lane C: PCR of cDNA using primers 2 and 4 Lane D: PCR of cDNA using primers 1 and 4 2 3 2 kb 0.5 kb 2 kb 0.5 kb 1 kb 2.5 kb 1 kb 10 9 8 7 6 5 4 3 2 A B C D 4

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter19: Genomes And Proteomes

Section: Chapter Questions

Problem 1TYK

See similar textbooks

Similar questions

Put the following tasks in the order they would occur during a DNA cloning experiment. a. using DNA ligase to seal DNA fragments into vectors b. using a probe to identify a clone in the library c. sequencing the DNA of the clone d. making a DNA library of clones e. cutting genomic DNA with restriction enzymes
What is the most challenging issue facing genome sequencing? a. the inability to develop fast and accurate sequencing techniques b. the ethics of using information from genomes at the individual level c. the availability and stability of DNA d. all of the above
Which of the following best describes the process of DNA sequencing? a. DNA is separated on a gel, and the different bands are labeled with fluorescent nucleotides and scanned with a laser. b. A laser is used to fluorescently label the nucleotides present within the DNA, the DNA is run on a gel, and then the DNA is broken into fragments. c. Nucleotides are scanned with a laser and incorporated into the DNA that has been separated on a gel, and then the DNA is amplified with PCR. d. Fragments of DNA are produced in a reaction that labels them with any of four different fluorescent dyes, and the fragments then are run on a gel and scanned with a laser. e. DNA is broken down into its constituent nucleotides, and the nucleotides are then run on a gel and purified with a laser.
Figure 17.7 You are working in a molecular biology lab and, unbeknownst to you, your lab partner left the foreign genomic DNA that you are planning to clone on the lab bench overnight instead of storing it in the freezer. As a result, it was degraded by nucleases, but still used in the experiment. The plasmid, on the other hand, is fine. What results would you expect from your molecular cloning experiment? There will be no colonies on the bacterial plate. There will be blue colonies only. There will be blue and white colonies. The will be white colonies only.
Transcribe and translate the following DNA sequence (nontemplate strand): 5’-ATGGCCGGTTATTAAGCA-3’
Cloning Genes Is a Multistep Process The following DNA sequence contains a six-base sequence that is a recognition and cutting site for a restriction enzyme. What is this sequence? Which enzyme will cut this sequence? (See Figure 13.5 for help.) 5 CCGAGGAAGCTTAC 3 3 GGCTCCTTCGAATG 5
You learned in the chapter that an STR locus is a locus where alleles differ in the number of copies of a short, tandemly repeated DNA sequence. PCR is used to determine the number of alleles present, as shown by the size of the DNA fragment amplified. In the Figure below are the results of PCR analysis for STR alleles at a locus where the repeat unit length is 9 bp, and alleles are known that have 5 to 11 copies of the repeat. Given the STR alleles present in the adults, state whether each of the four juveniles could or could not be an off-spring of those two adults. Explain your answers.
Discuss Concepts A forensic scientist obtained a small DNA sample from a crime scene. In order to examine the sample, he increased its quantity by cycling the sample through the polymerase chain reaction. He estimated that there were 50,000 copies of the DNA in his original sample. Derive a simple formula and calculate the number of copies he will have after15 cycles of the PCR.
What Are Clones? Cloning is a general term used for whole organisms and DNA sequences. Define what we mean when we say we have a clone.
Why are antibiotic resistance markers such as ampR important components of bacterial plasmid cloning vectors? a. The plasmid must have resistance to accept DNA inserts. b. They allow the detection of plasmids that contain an inserted DNA fragment. c. They ensure the presence of the ori site. d. They ensure that the plasmid can be cut by a restriction enzyme. e. They allow identification of bacteria that have taken up a plasmid.
Describe two of the applications for genome mapping.
What information and materials are needed to amplify region of DNA using PCR?