Figure 36.10 Agglutination Tests. (a) Tube agglutination test for determining antibody titer. The titer in this example is 160 because there is no agglutination in the next tube in the dilution series (1/320). The blue in the dilution tubes indicates the presence of the patient’s serum. (b) A microtiter plate illustrating hemagglutination. The antibody is placed in the wells (rows 1–10). Positive controls (row 11) and negative controls (row 12) are included. Red blood cells are added to each well. If sufficient antibody is present to agglutinate the cells, they sink as a mat to the bottom of the well. If insufficient antibody is present, they form a pellet at the bottom.
What is the titer for the antibody tested in row E?
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Prescott's Microbiology
- You have 4 ml of an antigen solution, how would you prepare a 3-fold dilution series such that you will have at least 2 ml of each dilution? Please draw a picture and explain, I don't understand what it means by 3-fold. Please explain, no copy paste asaparrow_forwardYou have 4 ml of an antigen solution, how would you prepare a 3-fold dilution series such that you will have at least 2 ml of each dilution? Please draw a picture and explain, I don't understand what it means by 3-fold.arrow_forward7 mL of a 1:50 dilution is needed to run a specific serological test. How much serum and how much diluent are needed to make this dilution? A 1:5 dilution of patient serum is necessary to run a serological test. There is 0.2 mL of serum that can be used. What amount of diluent is necessary to make this dilution using all of the serum?arrow_forward
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