Prescott's Microbiology
10th Edition
ISBN: 9781259281594
Author: Joanne Willey, Linda Sherwood Adjunt Professor Lecturer, Christopher J. Woolverton Professor
Publisher: McGraw-Hill Education
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Chapter 36.4, Problem 4RIA
Summary Introduction
To determine: The two types of ELISA methods and their working.
Introduction: ELISA which is also called Enzyme-Linked immune-sorbent Assay is the technique in which an enzyme-linked indicator antibody is used to visualize antigen-antibody reactions. This technique relies on a solid surface like plastic microtiter plate which is used for adsorption of reactants.
Summary Introduction
To describe: A chromogen.
Introduction: ELISA which is also called enzyme immunoassay (EIA) is the technique in which an enzyme-linked indicator antibody is used to visualize antigen-antibody reactions. This technique relies on a solid surface like plastic microtiter plate which is used for adsorption of reactants.
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Chapter 36 Solutions
Prescott's Microbiology
Ch. 36.3 - You have detected aerobic, Gram-positive cocci...Ch. 36.3 - Prob. 2MICh. 36.3 - Prob. 1RIACh. 36.3 - Describe in general how biochemical tests are used...Ch. 36.3 - Prob. 3RIACh. 36.3 - Prob. 4RIACh. 36.3 - Describe a dichotomous key that could be used to...Ch. 36.3 - How can nucleic acidbased detection methods be...Ch. 36.3 - How can a suspect bacterium be fingerprinted?Ch. 36.4 - Prob. 1MI
Ch. 36.4 - Figure 36.10 Agglutination Tests. (a) Tube...Ch. 36.4 - Prob. 3MICh. 36.4 - Prob. 4MICh. 36.4 - Prob. 1RIACh. 36.4 - Prob. 2RIACh. 36.4 - Prob. 3RIACh. 36.4 - Prob. 4RIACh. 36.4 - Prob. 5RIACh. 36.4 - Name two types of immunodiffusion tests and...Ch. 36.4 - Prob. 7RIACh. 36.4 - Prob. 8RIACh. 36.4 - Prob. 9RIACh. 36 - As more ways of identifying the characteristics of...Ch. 36 - Prob. 2CHICh. 36 - ELISA tests usually use a primary and secondary...Ch. 36 - Legionella pneumophila is a bacterium that is...Ch. 36 - Prob. 5CHI
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- What is the principle behind Liebermann-Burchard test? And What is the clinical significance of Liebermann-Burchard test?arrow_forwardWhat is the role of the substrate? Is it really necessary to add substrates in ELISA? Why, or why not.arrow_forwardYou are running your first ELISA and make some mistakes. What would happen if each of the following happened in isolation? Would the mistake inherently make the data unusable? (Tip: You might find the trouble shooting table from an ELISA protocol helpful, one is posted to ICON) a) You only add 50ul instead of 100 ul of both standards and experimental samples b) You use the same pipette tips to add the HRP-detection antibody and TMB substrate. c) You forget about your plate after adding your sample to a pre-coated plate and it incubates for an extra 4 hours. d) You forget about your plate after adding the TMB substrate and it incubates for an extra 4 hours. e) After adding the HRP-Detection antibody, you are so excited to see the result, you decide to wash just once and then add the TMB reagent.arrow_forward
- Explain the mechanisms of the three types of ELISA we discussed in lab: 1) direct, 2) indirect, 3) sandwich. Which type of ELISA did we do in lab? What is one advantage of this kind of ELISA?arrow_forwardWrite down basic principle of ELISA and its main steps? Write names of different types of ELISA. (Subject: Biotechnology)arrow_forwardIn relation to ELISA technique, fill in the missing information in the figure below: 1- 2- 3- 4-arrow_forward
- a) did the positive and negative controls work correctly in this ELISA? Explain. B) in the sample wells( wells3-7) some are darker blue than others. Explain what happened here and how you'd interpret the results?arrow_forwardIn general, what is the purpose of washing the red blood cell prior to testing? What are some of the tests that require washing of the red blood cells? What are the important considerations that you have to keep in mind in performing blood typing? Why is FORWARD typing only the screening procedure, not the confirmatory one? Why is REVERSE typing only the confirmatory procedure?arrow_forwardExplain what is the agglutination reaction: WHAT agglutinates with what? Agglutination is the positive or negative result? WHY?arrow_forward
- Does TGF-β treatment cause cells to grow more or less in the soft-agar assay? (a) More, (b) Lessarrow_forwardWhat is the point of doing a modified Furter-Meyer Test? What is the underlying idea of this test? How did you figure out that this test had a positive result?arrow_forwardExplain how PCR/OLA (polymerase chain reaction/oligonucleotide ligation assay) can be used in the diagnosis of sickle cell disorder . Would you recommend this method for routine diagnosis of sickle cell disorder? Explainarrow_forward
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