The reason for the significance of reporting the serotype of a rotavirus infection. Introduction: Rotaviruses belong to Reoviridae and are the most common causative agent for diarrheal diseases prevalent in the children. Rotavirus detection can be done by electron microscopy (EM), ELISA (enzyme-linked immunosorbent assay), polyacrylamide gel electrophoresis (PAGE) or the reverse transcription-polymerase chain reaction (RT-PCR).

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Answer 1

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Chapter 36, Problem 5CHI

Summary Introduction

To determine: The reason for the significance of reporting the serotype of a rotavirus infection.

Introduction: Rotaviruses belong to Reoviridae and are the most common causative agent for diarrheal diseases prevalent in the children. Rotavirus detection can be done by electron microscopy (EM), ELISA (enzyme-linked immunosorbent assay), polyacrylamide gel electrophoresis (PAGE) or the reverse transcription-polymerase chain reaction (RT-PCR).

Summary Introduction

To determine: The way in which PCR-ELISA differ from PCR.

Introduction: Rotaviruses belong to Reoviridae and are the most common causative agent for diarrheal diseases prevalent in the children. Rotavirus detection can be done by electron microscopy (EM), ELISA (enzyme-linked immunosorbent assay), polyacrylamide gel electrophoresis (PAGE) or the reverse transcription-polymerase chain reaction (RT-PCR).

Summary Introduction

To determine: The way in which PCR-ELISA differ from regular ELISA.

Introduction: Rotaviruses belong to Reoviridae and are the most common causative agent for diarrheal diseases prevalent in the children. Rotavirus detection can be done by electron microscopy (EM), ELISA (enzyme-linked immunosorbent assay), polyacrylamide gel electrophoresis (PAGE) or the reverse transcription-polymerase chain reaction (RT-PCR).

Summary Introduction

To determine: The reason for the PCR-ELISA for being better than the currently used typing PCR protocol.

Introduction: Rotaviruses belong to Reoviridae and are the most common causative agent for diarrheal diseases prevalent in the children. Rotavirus detection can be done by electron microscopy (EM), ELISA (enzyme-linked immunosorbent assay), polyacrylamide gel electrophoresis (PAGE) or the reverse transcription-polymerase chain reaction (RT-PCR).

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NOTE: explain in a paragraph form How does PCR help in the identification of the virus from patients?

Discuss the principles behind the use of the following techniques in the diagnosis of viral infections, providing examples of viruses diagnosed using these methods 5..1 ELISA 5.2 Restriction Fragment Length Polymorphism 5.3 Polymerase chain reaction

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Answer 2

Question

Chapter 36, Problem 5CHI

Summary Introduction

To determine: The reason for the significance of reporting the serotype of a rotavirus infection.

Introduction: Rotaviruses belong to Reoviridae and are the most common causative agent for diarrheal diseases prevalent in the children. Rotavirus detection can be done by electron microscopy (EM), ELISA (enzyme-linked immunosorbent assay), polyacrylamide gel electrophoresis (PAGE) or the reverse transcription-polymerase chain reaction (RT-PCR).

Summary Introduction

To determine: The way in which PCR-ELISA differ from PCR.

Introduction: Rotaviruses belong to Reoviridae and are the most common causative agent for diarrheal diseases prevalent in the children. Rotavirus detection can be done by electron microscopy (EM), ELISA (enzyme-linked immunosorbent assay), polyacrylamide gel electrophoresis (PAGE) or the reverse transcription-polymerase chain reaction (RT-PCR).

Summary Introduction

To determine: The way in which PCR-ELISA differ from regular ELISA.

Introduction: Rotaviruses belong to Reoviridae and are the most common causative agent for diarrheal diseases prevalent in the children. Rotavirus detection can be done by electron microscopy (EM), ELISA (enzyme-linked immunosorbent assay), polyacrylamide gel electrophoresis (PAGE) or the reverse transcription-polymerase chain reaction (RT-PCR).

Summary Introduction

To determine: The reason for the PCR-ELISA for being better than the currently used typing PCR protocol.

Introduction: Rotaviruses belong to Reoviridae and are the most common causative agent for diarrheal diseases prevalent in the children. Rotavirus detection can be done by electron microscopy (EM), ELISA (enzyme-linked immunosorbent assay), polyacrylamide gel electrophoresis (PAGE) or the reverse transcription-polymerase chain reaction (RT-PCR).

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Answer 3

Question

Chapter 36, Problem 5CHI

Summary Introduction

To determine: The reason for the significance of reporting the serotype of a rotavirus infection.

Introduction: Rotaviruses belong to Reoviridae and are the most common causative agent for diarrheal diseases prevalent in the children. Rotavirus detection can be done by electron microscopy (EM), ELISA (enzyme-linked immunosorbent assay), polyacrylamide gel electrophoresis (PAGE) or the reverse transcription-polymerase chain reaction (RT-PCR).

Summary Introduction

To determine: The way in which PCR-ELISA differ from PCR.

Introduction: Rotaviruses belong to Reoviridae and are the most common causative agent for diarrheal diseases prevalent in the children. Rotavirus detection can be done by electron microscopy (EM), ELISA (enzyme-linked immunosorbent assay), polyacrylamide gel electrophoresis (PAGE) or the reverse transcription-polymerase chain reaction (RT-PCR).

Summary Introduction

To determine: The way in which PCR-ELISA differ from regular ELISA.

Introduction: Rotaviruses belong to Reoviridae and are the most common causative agent for diarrheal diseases prevalent in the children. Rotavirus detection can be done by electron microscopy (EM), ELISA (enzyme-linked immunosorbent assay), polyacrylamide gel electrophoresis (PAGE) or the reverse transcription-polymerase chain reaction (RT-PCR).

Summary Introduction

To determine: The reason for the PCR-ELISA for being better than the currently used typing PCR protocol.

Introduction: Rotaviruses belong to Reoviridae and are the most common causative agent for diarrheal diseases prevalent in the children. Rotavirus detection can be done by electron microscopy (EM), ELISA (enzyme-linked immunosorbent assay), polyacrylamide gel electrophoresis (PAGE) or the reverse transcription-polymerase chain reaction (RT-PCR).

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Answer 4

Question

Chapter 36, Problem 5CHI

Summary Introduction

To determine: The reason for the significance of reporting the serotype of a rotavirus infection.

Introduction: Rotaviruses belong to Reoviridae and are the most common causative agent for diarrheal diseases prevalent in the children. Rotavirus detection can be done by electron microscopy (EM), ELISA (enzyme-linked immunosorbent assay), polyacrylamide gel electrophoresis (PAGE) or the reverse transcription-polymerase chain reaction (RT-PCR).

Summary Introduction

To determine: The way in which PCR-ELISA differ from PCR.

Introduction: Rotaviruses belong to Reoviridae and are the most common causative agent for diarrheal diseases prevalent in the children. Rotavirus detection can be done by electron microscopy (EM), ELISA (enzyme-linked immunosorbent assay), polyacrylamide gel electrophoresis (PAGE) or the reverse transcription-polymerase chain reaction (RT-PCR).

Summary Introduction

To determine: The way in which PCR-ELISA differ from regular ELISA.

Introduction: Rotaviruses belong to Reoviridae and are the most common causative agent for diarrheal diseases prevalent in the children. Rotavirus detection can be done by electron microscopy (EM), ELISA (enzyme-linked immunosorbent assay), polyacrylamide gel electrophoresis (PAGE) or the reverse transcription-polymerase chain reaction (RT-PCR).

Summary Introduction

To determine: The reason for the PCR-ELISA for being better than the currently used typing PCR protocol.

Introduction: Rotaviruses belong to Reoviridae and are the most common causative agent for diarrheal diseases prevalent in the children. Rotavirus detection can be done by electron microscopy (EM), ELISA (enzyme-linked immunosorbent assay), polyacrylamide gel electrophoresis (PAGE) or the reverse transcription-polymerase chain reaction (RT-PCR).

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Answer 5

Chapter 36, Problem 5CHI

Summary Introduction

To determine: The reason for the significance of reporting the serotype of a rotavirus infection.

Introduction: Rotaviruses belong to Reoviridae and are the most common causative agent for diarrheal diseases prevalent in the children. Rotavirus detection can be done by electron microscopy (EM), ELISA (enzyme-linked immunosorbent assay), polyacrylamide gel electrophoresis (PAGE) or the reverse transcription-polymerase chain reaction (RT-PCR).

Summary Introduction

To determine: The way in which PCR-ELISA differ from PCR.

Introduction: Rotaviruses belong to Reoviridae and are the most common causative agent for diarrheal diseases prevalent in the children. Rotavirus detection can be done by electron microscopy (EM), ELISA (enzyme-linked immunosorbent assay), polyacrylamide gel electrophoresis (PAGE) or the reverse transcription-polymerase chain reaction (RT-PCR).

Summary Introduction

To determine: The way in which PCR-ELISA differ from regular ELISA.

Introduction: Rotaviruses belong to Reoviridae and are the most common causative agent for diarrheal diseases prevalent in the children. Rotavirus detection can be done by electron microscopy (EM), ELISA (enzyme-linked immunosorbent assay), polyacrylamide gel electrophoresis (PAGE) or the reverse transcription-polymerase chain reaction (RT-PCR).

Summary Introduction

To determine: The reason for the PCR-ELISA for being better than the currently used typing PCR protocol.

Introduction: Rotaviruses belong to Reoviridae and are the most common causative agent for diarrheal diseases prevalent in the children. Rotavirus detection can be done by electron microscopy (EM), ELISA (enzyme-linked immunosorbent assay), polyacrylamide gel electrophoresis (PAGE) or the reverse transcription-polymerase chain reaction (RT-PCR).

Answer 6

Chapter 36, Problem 5CHI

Summary Introduction

To determine: The reason for the significance of reporting the serotype of a rotavirus infection.

Introduction: Rotaviruses belong to Reoviridae and are the most common causative agent for diarrheal diseases prevalent in the children. Rotavirus detection can be done by electron microscopy (EM), ELISA (enzyme-linked immunosorbent assay), polyacrylamide gel electrophoresis (PAGE) or the reverse transcription-polymerase chain reaction (RT-PCR).

Answer 7

Chapter 36, Problem 5CHI

Summary Introduction

To determine: The reason for the significance of reporting the serotype of a rotavirus infection.

Introduction: Rotaviruses belong to Reoviridae and are the most common causative agent for diarrheal diseases prevalent in the children. Rotavirus detection can be done by electron microscopy (EM), ELISA (enzyme-linked immunosorbent assay), polyacrylamide gel electrophoresis (PAGE) or the reverse transcription-polymerase chain reaction (RT-PCR).

Answer 8

Summary Introduction

To determine: The way in which PCR-ELISA differ from PCR.

Introduction: Rotaviruses belong to Reoviridae and are the most common causative agent for diarrheal diseases prevalent in the children. Rotavirus detection can be done by electron microscopy (EM), ELISA (enzyme-linked immunosorbent assay), polyacrylamide gel electrophoresis (PAGE) or the reverse transcription-polymerase chain reaction (RT-PCR).

Answer 9

Summary Introduction

To determine: The way in which PCR-ELISA differ from PCR.

Introduction: Rotaviruses belong to Reoviridae and are the most common causative agent for diarrheal diseases prevalent in the children. Rotavirus detection can be done by electron microscopy (EM), ELISA (enzyme-linked immunosorbent assay), polyacrylamide gel electrophoresis (PAGE) or the reverse transcription-polymerase chain reaction (RT-PCR).

Answer 10

Summary Introduction

To determine: The way in which PCR-ELISA differ from regular ELISA.

Introduction: Rotaviruses belong to Reoviridae and are the most common causative agent for diarrheal diseases prevalent in the children. Rotavirus detection can be done by electron microscopy (EM), ELISA (enzyme-linked immunosorbent assay), polyacrylamide gel electrophoresis (PAGE) or the reverse transcription-polymerase chain reaction (RT-PCR).

Answer 11

Summary Introduction

To determine: The way in which PCR-ELISA differ from regular ELISA.

Introduction: Rotaviruses belong to Reoviridae and are the most common causative agent for diarrheal diseases prevalent in the children. Rotavirus detection can be done by electron microscopy (EM), ELISA (enzyme-linked immunosorbent assay), polyacrylamide gel electrophoresis (PAGE) or the reverse transcription-polymerase chain reaction (RT-PCR).

Answer 12

Summary Introduction

To determine: The reason for the PCR-ELISA for being better than the currently used typing PCR protocol.

Introduction: Rotaviruses belong to Reoviridae and are the most common causative agent for diarrheal diseases prevalent in the children. Rotavirus detection can be done by electron microscopy (EM), ELISA (enzyme-linked immunosorbent assay), polyacrylamide gel electrophoresis (PAGE) or the reverse transcription-polymerase chain reaction (RT-PCR).

Answer 13

Summary Introduction

To determine: The reason for the PCR-ELISA for being better than the currently used typing PCR protocol.

Introduction: Rotaviruses belong to Reoviridae and are the most common causative agent for diarrheal diseases prevalent in the children. Rotavirus detection can be done by electron microscopy (EM), ELISA (enzyme-linked immunosorbent assay), polyacrylamide gel electrophoresis (PAGE) or the reverse transcription-polymerase chain reaction (RT-PCR).

Answer 14

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Answer 16

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Answer 17

Ch. 36.3 - You have detected aerobic, Gram-positive cocci...Ch. 36.3 - Prob. 2MI Ch. 36.3 - Prob. 1RIA Ch. 36.3 - Describe in general how biochemical tests are used...Ch. 36.3 - Prob. 3RIA Ch. 36.3 - Prob. 4RIA Ch. 36.3 - Describe a dichotomous key that could be used to...Ch. 36.3 - How can nucleic acidbased detection methods be...Ch. 36.3 - How can a suspect bacterium be fingerprinted?Ch. 36.4 - Prob. 1MI

Solution Summary: The author explains the importance of reporting the serotype of a rotavirus infection. PCR-ELISA is an immunodetection technique that detects nucleic acid rather than protein.

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