Concept explainers
To determine: The reason for the significance of reporting the serotype of a rotavirus infection.
Introduction: Rotaviruses belong to Reoviridae and are the most common causative agent for diarrheal diseases prevalent in the children. Rotavirus detection can be done by electron microscopy (EM), ELISA (enzyme-linked immunosorbent assay), polyacrylamide gel electrophoresis (PAGE) or the reverse transcription-polymerase chain reaction (RT-PCR).
To determine: The way in which PCR-ELISA differ from PCR.
Introduction: Rotaviruses belong to Reoviridae and are the most common causative agent for diarrheal diseases prevalent in the children. Rotavirus detection can be done by electron microscopy (EM), ELISA (enzyme-linked immunosorbent assay), polyacrylamide gel electrophoresis (PAGE) or the reverse transcription-polymerase chain reaction (RT-PCR).
To determine: The way in which PCR-ELISA differ from regular ELISA.
Introduction: Rotaviruses belong to Reoviridae and are the most common causative agent for diarrheal diseases prevalent in the children. Rotavirus detection can be done by electron microscopy (EM), ELISA (enzyme-linked immunosorbent assay), polyacrylamide gel electrophoresis (PAGE) or the reverse transcription-polymerase chain reaction (RT-PCR).
To determine: The reason for the PCR-ELISA for being better than the currently used typing PCR protocol.
Introduction: Rotaviruses belong to Reoviridae and are the most common causative agent for diarrheal diseases prevalent in the children. Rotavirus detection can be done by electron microscopy (EM), ELISA (enzyme-linked immunosorbent assay), polyacrylamide gel electrophoresis (PAGE) or the reverse transcription-polymerase chain reaction (RT-PCR).
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Prescott's Microbiology
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