Prescott's Microbiology
10th Edition
ISBN: 9781259281594
Author: Joanne Willey, Linda Sherwood Adjunt Professor Lecturer, Christopher J. Woolverton Professor
Publisher: McGraw-Hill Education
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Chapter 36.4, Problem 3MI
Summary Introduction
To determine: The reason for the use of indirect ELISA to test for HIV.
Introduction: ELISA which is also called enzyme-linked immune-sorbent Assay is the technique in which an enzyme-linked indicator antibody is used to visualize antigen-antibody reactions. Indirect and direct immune-sorbent assay are the two types of ELISA. The indirect immune-sorbent assay detects antibodies while direct immune-sorbent assay detects antigens.
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Chapter 36 Solutions
Prescott's Microbiology
Ch. 36.3 - You have detected aerobic, Gram-positive cocci...Ch. 36.3 - Prob. 2MICh. 36.3 - Prob. 1RIACh. 36.3 - Describe in general how biochemical tests are used...Ch. 36.3 - Prob. 3RIACh. 36.3 - Prob. 4RIACh. 36.3 - Describe a dichotomous key that could be used to...Ch. 36.3 - How can nucleic acidbased detection methods be...Ch. 36.3 - How can a suspect bacterium be fingerprinted?Ch. 36.4 - Prob. 1MI
Ch. 36.4 - Figure 36.10 Agglutination Tests. (a) Tube...Ch. 36.4 - Prob. 3MICh. 36.4 - Prob. 4MICh. 36.4 - Prob. 1RIACh. 36.4 - Prob. 2RIACh. 36.4 - Prob. 3RIACh. 36.4 - Prob. 4RIACh. 36.4 - Prob. 5RIACh. 36.4 - Name two types of immunodiffusion tests and...Ch. 36.4 - Prob. 7RIACh. 36.4 - Prob. 8RIACh. 36.4 - Prob. 9RIACh. 36 - As more ways of identifying the characteristics of...Ch. 36 - Prob. 2CHICh. 36 - ELISA tests usually use a primary and secondary...Ch. 36 - Legionella pneumophila is a bacterium that is...Ch. 36 - Prob. 5CHI
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- Why do you coat the elisa plate with albumin?arrow_forwardYou performed an Elisa test in the lab to detect antibody against HIV in the patient's serum. The test was found to be 96% sensitive. What does that mean? A western blot was used on the same sample to confirm, and the test was found to be 99% specific. What does that mean?arrow_forwardwhich combination of tests would be the most reliable and practical in diagonsing an HIV infection? a)Elisa, Oraquick adance b)P 24 antiboy, Western blot c)Bombo Antigen/antibody quick test, PCR testing d)oraquick advance antibody test, ELISA e) Western blot, PCR testingarrow_forward
- The Recombinant Factor C endotoxin test uses blood from horseshoe crabs. true or false?arrow_forwardWhy is the ELISA test used to screen human serum for the AIDSvirus, while the Western blot procedure is used only as the confirmation test?arrow_forwardhow does teh ELISA help determined who the initial disease carrier is. Could you use ELISA to narrow it down to just one carrier? Why or why not?arrow_forward
- Explain the limitations of the ELISA for HIV testing. What might cause a false positive result? What might cause a false negative result?arrow_forwardWhat are ELISA assays used for in labs? Give at least three examples.arrow_forwardIn relation to ELISA technique, fill in the missing information in the figure below: 1- 2- 3- 4-arrow_forward
- If you forgot to wash the wells after you added the secondary antibody, what would you expect to see in each of the four rows of your ELISA?arrow_forwardWhat is the effect of not adding the primary antibody to the ELISA reaction?arrow_forwardIs rapid antibody testing a form of ELISA? Explain your answer. How is an ELISA different from rapid antibody testing? Describe how antibodies are used to detect the presence of a specific disease.arrow_forward
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