Prescott's Microbiology
10th Edition
ISBN: 9781259281594
Author: Joanne Willey, Linda Sherwood Adjunt Professor Lecturer, Christopher J. Woolverton Professor
Publisher: McGraw-Hill Education
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Chapter 36.3, Problem 3RIA
Summary Introduction
To determine: The advantages of using monoclonal antibody immunofluorescence in the identification of viruses.
Introduction: The antibodies made from same immune cells that are clones of a unique parent cell are called monoclonal antibodies (mAb). These antibodies are monovalent which means they have affinity towards a single type of antibody and thus they interact with the same epitope.
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Chapter 36 Solutions
Prescott's Microbiology
Ch. 36.3 - You have detected aerobic, Gram-positive cocci...Ch. 36.3 - Prob. 2MICh. 36.3 - Prob. 1RIACh. 36.3 - Describe in general how biochemical tests are used...Ch. 36.3 - Prob. 3RIACh. 36.3 - Prob. 4RIACh. 36.3 - Describe a dichotomous key that could be used to...Ch. 36.3 - How can nucleic acidbased detection methods be...Ch. 36.3 - How can a suspect bacterium be fingerprinted?Ch. 36.4 - Prob. 1MI
Ch. 36.4 - Figure 36.10 Agglutination Tests. (a) Tube...Ch. 36.4 - Prob. 3MICh. 36.4 - Prob. 4MICh. 36.4 - Prob. 1RIACh. 36.4 - Prob. 2RIACh. 36.4 - Prob. 3RIACh. 36.4 - Prob. 4RIACh. 36.4 - Prob. 5RIACh. 36.4 - Name two types of immunodiffusion tests and...Ch. 36.4 - Prob. 7RIACh. 36.4 - Prob. 8RIACh. 36.4 - Prob. 9RIACh. 36 - As more ways of identifying the characteristics of...Ch. 36 - Prob. 2CHICh. 36 - ELISA tests usually use a primary and secondary...Ch. 36 - Legionella pneumophila is a bacterium that is...Ch. 36 - Prob. 5CHI
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- Explain how PCR/OLA (polymerase chain reaction/oligonucleotide ligation assay) can be used in the diagnosis of sickle cell disorder . Would you recommend this method for routine diagnosis of sickle cell disorder? Explainarrow_forwardWhich of the following are true regarding assay attributes? List all that are true. a) Accuracy represents the closeness of the assay result to the “true value”. b) Robustness represents the ability of a method to distinguish between the analyte and similar components. c) Precision is determined by replicate analysis of a reference standard or well-characterized material. d) A robust assay would have a very narrow range of acceptable assay conditions. For those that were not true in question 1, correct the statement(s).arrow_forwardSouthern blotting technique is used ina) Monoclonal antibody productionb) In vitro culturec) Genetic finger printingd) Polymerase chain reactionarrow_forward
- 4) What is the advantage of cryo-sectioning for antibody staining methods? 5) When preparing coverslips with cultured cells for immunofluorescence why is it necessary to coat coverslips with poly-L-lysine or gelatin?arrow_forwardIn relation to immunotechnology, answer the following: Give an example of murine Monocolonal Antibody produced by hybridoma technology?arrow_forwardWhich of the following is TRUE about immunofluorescence microscopy where we localize proteins using fluorescently tagged antibodies? Pick the best answer: We can use it to resolve 2 points that are 10 nanometers apart. The emission wavelength of the fluorophore is typically shorter than the excitation wavelength. The resolution can be further improved with increased magnification. All else being equal, a fluorophore that emits red light will give better resolution than a fluorophore that emits green light. Based on what we discussed in class, we can use the technique on live samples therefore enabling us to see the dynamic movement of cytosolic proteins in living cells. None of the abovearrow_forward
- What is an advantage of using an ELISA instead of a protein microarray to study a proteome? What is a disadvantage?arrow_forwardWhat are the advantages and disadvantages of agglutinationtests versus fluorescent antibody assays? How are the latter usedto identify specific cells in complex mixtures, such as blood?arrow_forwardOutline the principle behind the following techniques: 1. Immunofixation 2. Southern blotting 3. Ion exchange chromatograph 4. SDS-PAGE.arrow_forward
- 1) Which technique is best suited to determining which genes are activated in a bacterium during infection while causing disease in a person. a) SDS-page b) microarray analysis c) RFLP analysis d) clone library analysis 2)Which of the following is not an application of PCR? a) Determine if two people are related. b) Identify a bacterial pathogen in a patient sample. c) Determine the gene sequence of the gene that codes for a bacterial enterotoxin. d) These are all applications of PCR.arrow_forwardWhat is the application of antibodies in biotechnology? Please answer at your own words.arrow_forwardDefine about immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH) assays ?arrow_forward
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