Prescott's Microbiology
10th Edition
ISBN: 9781259281594
Author: Joanne Willey, Linda Sherwood Adjunt Professor Lecturer, Christopher J. Woolverton Professor
Publisher: McGraw-Hill Education
expand_more
expand_more
format_list_bulleted
Videos
Question
Chapter 36.3, Problem 2MI
Summary Introduction
To determine: The type of immunofluorescence which would be most likely to be used to test a clinical sample for the presence of an intracellular pathogen such as hepatitis C virus.
Introduction: ELISA which is also called Enzyme-Linked immune-sorbent Assay is the technique in which an enzyme-linked indicator antibody is used to visualize antigen-antibody reactions. Indirect and direct immune-sorbent assay are the two types of ELISA. The indirect immune-sorbent assay detects antibodies while direct immune-sorbent assay detects antigens.
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
Note that it is not appropriate to self-diagnose outside of a medical context and this is a completely hypothetical scenario.
Imagine you have a rash on your foot. You're concerned that it's an infection and inoculate a sample onto an agar plate. You wonder, How can I figure out whether the pathogen is a bacterium vs a eukaryote?
You decide to use lab supplies to get a basic understanding of the pathogen. Be specific about what tests you use and what you expect the results to be. Limit yourself to experiments we could do in our lab.
What is one experiment you could do, involving culturing the organism?
Note that it is not appropriate to self-diagnose outside of a medical context and this is a completely hypothetical scenario.
Imagine you have a rash on your foot. You're concerned that it's an infection and inoculate a sample onto an agar plate. You wonder, How can I figure out whether the pathogen is a bacterium vs a eukaryote?
You decide to use lab supplies to get a basic understanding of the pathogen. Be specific about what tests you use and what you expect the results to be. Limit yourself to experiments we could do in our lab.
What is a procedure you could do, involving making a slide of the organism?
What are the four steps of an ELISA (enzyme-linked immunosorbent assay) protocol?
Chapter 36 Solutions
Prescott's Microbiology
Ch. 36.3 - You have detected aerobic, Gram-positive cocci...Ch. 36.3 - Prob. 2MICh. 36.3 - Prob. 1RIACh. 36.3 - Describe in general how biochemical tests are used...Ch. 36.3 - Prob. 3RIACh. 36.3 - Prob. 4RIACh. 36.3 - Describe a dichotomous key that could be used to...Ch. 36.3 - How can nucleic acidbased detection methods be...Ch. 36.3 - How can a suspect bacterium be fingerprinted?Ch. 36.4 - Prob. 1MI
Ch. 36.4 - Figure 36.10 Agglutination Tests. (a) Tube...Ch. 36.4 - Prob. 3MICh. 36.4 - Prob. 4MICh. 36.4 - Prob. 1RIACh. 36.4 - Prob. 2RIACh. 36.4 - Prob. 3RIACh. 36.4 - Prob. 4RIACh. 36.4 - Prob. 5RIACh. 36.4 - Name two types of immunodiffusion tests and...Ch. 36.4 - Prob. 7RIACh. 36.4 - Prob. 8RIACh. 36.4 - Prob. 9RIACh. 36 - As more ways of identifying the characteristics of...Ch. 36 - Prob. 2CHICh. 36 - ELISA tests usually use a primary and secondary...Ch. 36 - Legionella pneumophila is a bacterium that is...Ch. 36 - Prob. 5CHI
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- A suspected pathogen is observed from the blood of a diseased mouse. An investigation of the other mice in the area indicates that it is not present in healthy mice. With Koch's postulates in mind, what is the next step to determine whether or not this suspected pathogen is the cause of disease in the mouse? a) Grow the suspected pathogen in a pure culture Ob) Re-isolate the suspected pathogen and show that it is the same as the original pathogen c) Inoculate a healthy mouse with the suspected pathogen O d) Determine whether the organism in the pure culture is the same one as in the original samplearrow_forwardWhich of the following is TRUE about immunofluorescence microscopy where we localize proteins using fluorescently tagged antibodies? Pick the best answer: We can use it to resolve 2 points that are 10 nanometers apart. The emission wavelength of the fluorophore is typically shorter than the excitation wavelength. The resolution can be further improved with increased magnification. All else being equal, a fluorophore that emits red light will give better resolution than a fluorophore that emits green light. Based on what we discussed in class, we can use the technique on live samples therefore enabling us to see the dynamic movement of cytosolic proteins in living cells. None of the abovearrow_forwardAll of the following are used in a direct ELISA, except: A) Primary enzyme linked antibody B) Substrate C) Secondary enzyme linked antibody D) Primary antibodyarrow_forward
- In the description of the preparation of an OR for surgery, bleach rather that alcohol was used to disinfect after delivery of a baby from an HIV-infected mother. What is the mode of killing for both bleach and alcohol? Why was bleach used instead of alcohol?arrow_forwardWhy is that bacterial slide agglutination technique important in diagnostic procedure?arrow_forwardCan hand sanitizer kill bacteria?arrow_forward
- There are so many microbes in a single mL of culture, it is very difficult to perform one dilution to produce countable cells. Microbiologists need to perform a dilution series, where multiple dilutions are performed in sequence to arrive at the correct dilution. Dilutions are cumulative. Multiple the series of dilutions together to find the final dilution value. If 3 serial dilutions are performed, each with a value of 0.01, what is the cumulative dilution? Express your answer as an exponent, e.g. 0.1 would be 1e-1 and 0.01 would be 1e-2arrow_forward1) What are you testing in the picture above? 2) How would you interpret this test? 3) What is the basis for this type of test?arrow_forwardA negative result with MPO stain would be seen with what cell type? Question 5 options: A) neutrophils B) lymphocytes C) eosinophils D) basophilsarrow_forward
- One laboratory method to detect for COVID-19 infection is via real time RT-PCR. The process starts with nasopharyngeal or oropharyngeal swabbing to get a sample. Explain concisely how this technique generally works and how it can be used for testing.arrow_forwardin an indirect elisa procedure what enzyme is used?arrow_forwardDoes TGF-β treatment cause cells to grow more or less in the soft-agar assay? (a) More, (b) Lessarrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)BiologyISBN:9780134580999Author:Elaine N. Marieb, Katja N. HoehnPublisher:PEARSON
Biology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStax
Anatomy & PhysiologyBiologyISBN:9781259398629Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa StouterPublisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)BiologyISBN:9780815344322Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter WalterPublisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & PhysiologyBiologyISBN:9781260159363Author:Martin, Terry R., Prentice-craver, CynthiaPublisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)BiologyISBN:9781260231700Author:Sylvia S. Mader, Michael WindelspechtPublisher:McGraw Hill Education
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:PEARSON
Biology 2e
Biology
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:OpenStax
Anatomy & Physiology
Biology
ISBN:9781259398629
Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:9780815344322
Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:9781260159363
Author:Martin, Terry R., Prentice-craver, Cynthia
Publisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Biology
ISBN:9781260231700
Author:Sylvia S. Mader, Michael Windelspecht
Publisher:McGraw Hill Education
Haematology - Red Blood Cell Life Cycle; Author: Armando Hasudungan;https://www.youtube.com/watch?v=cATQFej6oAc;License: Standard youtube license