Which of the following is not a strength of using 16S rRNA for phylogenetic analyses? OA. It's cheap OB. It's easy to do C. It can be used to identify all the way down to the strain level OD. Both A & B OE. None of the above

Which of the following is FALSE of functional genomics? Select ALL that apply. RNA seq is a functional genomics approach CHIP seq is a functional genomics approach It seeks to elucidate the roles of genetic sequences of a given species Was invented by Carey Mullis Seeks to edit the DNA sequence of the organism Artificial selection is a functional genomics approach

This descriptor refers to the number of alignments expected to match the sequence you have by chance. E-value Query cover Accession number Ident Maximum score and Total score

Which of these is not a tool for comparing DNA sequences?

Why is it important to sequence positive clones derived from PCR cloning? Group of answer choices Errors could have been incorporated during restriction enzyme digestion and so it is important to verify that only the expected protein is produced.   Errors could have been incorporated during plasmid DNA extraction and so it is important to verify that only the expected protein is produced.   Errors could have been incorporated during PCR and so it is important to verify that only the expected protein is produced.   Errors could have been incorporated during cloning and so it is important to verify that only the expected protein is produced.

BOTTOM The sequence depicted on the gel is 5'-GTGATGTAG-3' The sequence depicted on the gel is 3'- GTGATGTAG -5' The sequence shown on the gel is the sequence of the template strand of the reaction The sequence shown on the gel is complementary to the template strand of the reaction The template strand of the reaction is antiparallel to the sequence shown on the gel The largest fragment shown on the gel is closest to the top The largest fragment shown on the gel is closest to the bottom Each of the fragments shown on the gel have a primer incorporated at their 5' end Each of the fragments shown on the gel have a primer incorporated at their 3' end The number of nucleotides in the shortest band on the gel is 21 The number of nucleotides in the longest band on the gel is 21 ick Save and Submit to save and submit. Cick Save All Answers to save all answers.

Which of the following statements is true of a Sanger sequencing reaction but not colony PCR?  Select all that apply. Must contain only a single primer ddNTPs are added Uses a heat stable DNA polymerase Requires a pair of primers that bind to opposite strands Requires temperature cycling

Which of the following is TRUE of next generation sequencing technology? It is required for CHIP-seq and RNA-seq It can be used to directly sequence RNA It has facilitated an explosion of publicly available genome sequences for eukaryotes and prokaryotes It is extremely expensive as compared to previous sequencing approaches Illumina is a next generation sequencing platform It can be used to sequence proteins (proteomics)

You're the geneticist on a team that's uncovered a Wooly Mammoth in the ice and you want to study the function of its hemoglobin protein. First, you need to PCR amplify the gene to be able to Sanger sequence it. What reference genome do you use to design primers? O The human genome because it's the best O The bat genome because bats are cool O The elephant genome because it likely has the most sequence similarity given evolutionary distance O The E. coli genome because they're such a great model organism O The yeast genome because they're small and they're a eukaryote

After you design the PCR primers, you run the PCR on fluid from the patient’s nasal swab. Next, you need to evaluate the results. PCR makes billions of copies of just one sequence in a sample. Since you know the sequence, you also know the length (number of nucleotides) of the region you copied. There are about 130 nucleotides in the N gene fragment. This is typically stated as 130 base pairs. How can you visualize DNA and estimate its size? Load the DNA sample into an agarose gel (similar in consistency to jello) and apply an electric current to the gel. The DNA is charged and will move through the gel. The longer the DNA fragment, the more slowly it moves. The DNA is visualized by adding a fluorescent dye to your sample that sticks to DNA. When you look at the gel under UV light, the DNA should glow. What is the charge of a DNA molecule? Based on #1, would you expect DNA to be drawn to the (+) or (-) electrode of the gel electrophoresis chamber?