You will use the following scenario to answer a group of 5 questions. You have isolated amicrobe from an environmental sample. The microbe has the ability to perform a newmetabolic reaction at a very low temperature, so you are excited that it could be a newspecies.You have shipped your samples off for sequencing and are now waiting for the results. Out ofcuriosity (and maybe boredom...) you decide to test your culture for the Catalase and Oxidaseenzymes. Upon testing your sample for catalase, you don't see any bubbles; however, you dosee a color change to purple during the Oxidase test. What results can you conclude fromthis?O Catalase-/ Oxidase +O Catalase +/ Oxidase +Catalase + / Oxidase-O Catalase / Oxidase -O None of the above

First, let's differentiate a catalase test from an oxidase test. Catalase Test checks whether a…

Answered: You will use the following scenario to…

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter7: Basic Clinical Microbiology

Section7.7: Bacterial Identification And Antibiotic Susceptibility Testing

Problem 3CS

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Similar questions

Which Is â€œBetter: NMR or MS? Compare and contrast mass spectrometry and NMR. in terms of their potential advantages and disadvantages for metabolomic analysis.
A scientist isolates a new species of prokaryote He notes that the specimen is a bacillus with a lipid bilayer and cell wall that stains positive for peptidoglycan. Its circular chromosome replicates from a single origin of replication. Is the specimen most likely an Archaea, a Gram-positive bacterium, or a Gram-negative bacterium? How do you know?
Sterile normal saline is used in most surgical procedures as an irrigation solution. During certain surgical procedures, such as those for malignancy, the surgeon may request that sterile water be used for irrigation instead of saline following excision of the cancerous mass. Why is normal saline usually used in healthy tissues?
What are the properties of F+, F-, and Hfr cells of E. coli?
Antibiotics and Protein Synthesis Antibiotics are molecules produced by microorganisms as defense mechanisms. The most effective antibiotics work by interfering with essential biochemical or reproductive processes. Many antibiotics block or disrupt one or more stages in protein synthesis. Some of these are mentioned here. Tetracyclines are a family of chemically related compounds used to treat several types of bacterial infections. Tetracyclines interfere with the initiation of translation. The tetracycline molecule attaches to the small ribosomal subunit and prevents binding of the tRNA anticodon during initiation. Both eukaryotic and prokaryotic ribosomes are sensitive to the action of tetracycline, but this antibiotic cannot pass through the plasma membrane of eukaryotic cells. Because tetracycline can enter bacterial cells to inhibit protein synthesis, it will stop bacterial growth, helping the immune system fight the infection. Streptomycin is used in hospitals to treat serious bacterial infections. It binds to the small ribosomal subunit but does not prevent initiation or elongation; however, it does affect the efficiency of protein synthesis. Binding of streptomycin changes the way mRNA codons interact with the tRNA. As a result, incorrect amino acids are incorporated into the growing polypeptide chain, producing nonfunctional proteins. In addition, streptomycin causes the ribosome to randomly fall off the mRNA, preventing the synthesis of complete proteins. Puromycin is not used clinically but has played an important role in studying the mechanism of protein synthesis in the research laboratory. The puromycin molecule is the same size and shape as a tRNA/amino acid complex. When puromycin enters the ribosome, it can be incorporated into a growing polypeptide chain, stopping further synthesis because no peptide bond can be formed between puromycin and an amino acid, causing the shortened polypeptide to fall off the ribosome. Chloramphenicol was one of the first broadspectrum antibiotics introduced. Eukaryotic cells are resistant to its actions, and it was widely used to treat bacterial infections. However, its use is limited to external applications and serious infections. Chloramphenicol destroys cells in the bone marrow, the source of all blood cells. In bacteria, this antibiotic binds to the large ribosomal subunit and inhibits the formation of peptide bonds. Another antibiotic, erythromycin, also binds to the large ribosomal subunit and inhibits the movement of ribosomes along the mRNA. Almost every step of protein synthesis can be inhibited by one antibiotic or another. Work on designing new synthetic antibiotics to fight infections is based on our knowledge of how the nucleotide sequence of mRNA is converted into the amino acid sequence of a protein. Questions Why is targeting protein synthesis an effective strategy for preventing infection?
Which of the following best describes the process of DNA sequencing? a. DNA is separated on a gel, and the different bands are labeled with fluorescent nucleotides and scanned with a laser. b. A laser is used to fluorescently label the nucleotides present within the DNA, the DNA is run on a gel, and then the DNA is broken into fragments. c. Nucleotides are scanned with a laser and incorporated into the DNA that has been separated on a gel, and then the DNA is amplified with PCR. d. Fragments of DNA are produced in a reaction that labels them with any of four different fluorescent dyes, and the fragments then are run on a gel and scanned with a laser. e. DNA is broken down into its constituent nucleotides, and the nucleotides are then run on a gel and purified with a laser.
Which of these statements is true? An antibiotic is any substance produced by a organism that is antagonistic to the growth of prokaryotes An antibiotic is any substance produced by a prokaryote that is antagonistic to the growth of other viruses An antibiotic is any substance produced by a prokaryote that is antagonistic to the growth of eukaryotic cells An antibiotic is any substance produced by a prokaryote that prevents growth of the same prokaryote.
In the experiments of Aery, MacLeod, and McCarty, what was the purpose of treating the transforming extract with enzymes?
In addition to providing yogurt with its unique flavor and texture, lactic acid-producing bacteria also provide which additional benefit during food production? Providing xenobiotics Lowering the pH to kill pathogenic bacteria Pasteurizing milk products Breaking down lactose for lactose-intolerant individuals
Synthetic compounds found in an organism but not normally produced or expected to be present in that organism are called _____________. pesticides bioi emediators recalcitrant compounds xenobiotics
What type of micrograph is shown below? Is the organism pictured prokaryotic or eukaryotic? How can you tell?

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