In an experiemnt with various temperatures (4°C, 22°C, 50°C and 70°C) to examine how temperature impacts peroxidase activity. State the null hypothesis and alternative hypothesis for this experiment. (Enzyme works best at 50°C) 2. Draw a graph showing relationship of absorbance over time in three cuvettes, (a) one with high amounts of enzyme, (b) one with low amounts of enzyme, and (c) one with medium amounts of enzyme. Assume that the amount of substrate is the same in all three, as is the amount of the dye, guaiacol. Make sure that you have all the components of the graph including axes titles, units of measurement, legends, etc. 3. A group of researchers varied the concentrations of Fe2+ cofactors added in the solution in which a Hydrogen peroxide + peroxidase reaction took place. They added the cofactor at concentrations = 0.5 ml, 0.4 ml, 0.3 ml, 0.2 ml, 0.1 ml. A. Draw a graph depicting expected results for this experiment. Make sure that all the components of the graph including axes titles, units of measurement, legends, etc. 4. Pepsin is a stomach enzyme that serves to digest proteins. According to the statement, what would most likely be the optimum pH for this enzyme? Explain.
Enzyme kinetics
In biochemistry, enzymes are proteins that act as biological catalysts. Catalysis is the addition of a catalyst to a chemical reaction to speed up the pace of the reaction. Catalysis can be categorized as either homogeneous or heterogeneous, depending on whether the catalysts are distributed in the same phase as that of the reactants. Enzymes are an essential part of the cell because, without them, many organic processes would slow down and thus will affect the processes that are important for cell survival and sustenance.
Regulation of Enzymes
A substance that acts as a catalyst to regulate the reaction rate in the living organism's metabolic pathways without itself getting altered is an enzyme. Most of the biological reactions and metabolic pathways in the living systems are carried out by enzymes. They are specific for their works and work in particular conditions. It maintains the best possible rate of reaction in the most stable state. The enzymes have distinct properties as they can proceed with the reaction in any direction, their particular binding sites, pH specificity, temperature specificity required in very few amounts.
- In an experiemnt with various temperatures (4°C, 22°C, 50°C and 70°C) to examine how temperature impacts peroxidase activity. State the null hypothesis and alternative hypothesis for this experiment. (Enzyme works best at 50°C)
2. Draw a graph showing relationship of absorbance over time in three cuvettes, (a) one with high amounts of enzyme, (b) one with low amounts of enzyme, and (c) one with medium amounts of enzyme. Assume that the amount of substrate is the same in all three, as is the amount of the dye, guaiacol. Make sure that you have all the components of the graph including axes titles, units of measurement, legends, etc.
3. A group of researchers varied the concentrations of Fe2+ cofactors added in the solution in which a Hydrogen peroxide + peroxidase reaction took place. They added the cofactor at concentrations = 0.5 ml, 0.4 ml, 0.3 ml, 0.2 ml, 0.1 ml.
A. Draw a graph depicting expected results for this experiment. Make sure that all the components of the graph including axes titles, units of measurement, legends, etc.
4. Pepsin is a stomach enzyme that serves to digest proteins. According to the statement, what would most likely be the optimum pH for this enzyme? Explain.
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