Biochemistry
6th Edition
ISBN: 9781305577206
Author: Reginald H. Garrett, Charles M. Grisham
Publisher: Cengage Learning
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Chapter 27, Problem 5P
Interpretation Introduction
Interpretation:
The value of concentration of ADP and AMP if the adenylate kinase reaction is at equilibrium.
Concept introduction:
Adenylate enzyme (also called ADK or myokinase) could be a phosphotransferase catalyst that catalyzes the interconversion of purine
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If intracellular [ATP] = 5 mM, [ADP] = 0.5 mM, and [Pi] = 1.0 mM, calculate the concentration of AMP at pH 7 and 25°C under the condition that the adenylate kinase reaction is at equilibrium.
1E
In terms of binding adenylate kinase, the Kd for ATP is ~50 M and the KI for GMP-PCP is ~50 nM. Explain how this may be possible in terms of molecular interactions. Name 4 different types of molecular interactions that may contribute to the increased binding affinity exhibited by GMP-PCP.
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=
20 nM. The rate of receptor-ligand complex formation with an
A receptor-ligand complex has a dissociation constant of Ka
added ligand concentration of 10 µM is 5 × 10³ s¯¹. What is the value of the reverse rate constant, k_₁ ?
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