EBK PRINCIPLES OF ANATOMY AND PHYSIOLOG
16th Edition
ISBN: 9781119662686
Author: DERRICKSON
Publisher: VST
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Chapter 21, Problem 2CTQ
Michael was brought into the emergency room suffering from a gunshot wound. He is bleeding profusely and exhibits the following: systolic blood pressure is 40 mmHg; weak pulse of 200 beats per minute; cool, pale, and clammy skin. Michael is not producing urine but is asking for water. He is confused and disoriented. What is his diagnosis and what, specifically, is causing these symptoms?
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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Chapter 21 Solutions
EBK PRINCIPLES OF ANATOMY AND PHYSIOLOG
Ch. 21 - Prob. 1CPCh. 21 - Prob. 2CPCh. 21 - Prob. 3CPCh. 21 - Prob. 4CPCh. 21 - Prob. 5CPCh. 21 - Prob. 6CPCh. 21 - Prob. 7CPCh. 21 - Prob. 8CPCh. 21 - Prob. 9CPCh. 21 - Prob. 10CP
Ch. 21 - Prob. 11CPCh. 21 - Prob. 12CPCh. 21 - Prob. 13CPCh. 21 - Prob. 14CPCh. 21 - Prob. 15CPCh. 21 - Prob. 16CPCh. 21 - Prob. 17CPCh. 21 - Prob. 18CPCh. 21 - Prob. 19CPCh. 21 - Prob. 20CPCh. 21 - Prob. 21CPCh. 21 - 22. Describe the types of shock and their causes...Ch. 21 - What is the purpose of systemic circulation?Ch. 21 - Prob. 24CPCh. 21 - Prob. 25CPCh. 21 - Prob. 26CPCh. 21 - Prob. 27CPCh. 21 - Prob. 28CPCh. 21 - Prob. 29CPCh. 21 - Prob. 30CPCh. 21 - Prob. 31CPCh. 21 - Prob. 32CPCh. 21 - Prob. 33CPCh. 21 - Prob. 34CPCh. 21 - Prob. 35CPCh. 21 - Prob. 36CPCh. 21 - Prob. 37CPCh. 21 - Prob. 38CPCh. 21 - Prob. 39CPCh. 21 - Prob. 40CPCh. 21 - 1. Kim Sung was told that her baby was born with a...Ch. 21 - 2. Michael was brought into the emergency room...Ch. 21 - Prob. 3CTQ
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