Biochemistry: Concepts and Connections (2nd Edition)
Biochemistry: Concepts and Connections (2nd Edition)
2nd Edition
ISBN: 9780134641621
Author: Dean R. Appling, Spencer J. Anthony-Cahill, Christopher K. Mathews
Publisher: PEARSON
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Chapter 2, Problem 9P

a. Suppose you wanted to make a buffer of exactly pH 7.00 using KH2PO4 and
Na2HPO4. If the final solution was 0.1 M in KH2PO4, what concentration of Na2HPO4 would you need?
b. Now assume you wish to make a buffer at the same pH, using the same substances, but want the total phosphate molarity ([HPO42- ]+[H2PO4-]) to equal 0.3. What concentrations of the KH2PO4 and Na2HPO4 would be required?

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Please help with this Mass Spectrometry Question. Thank you For the mass spec. shown in the attached image, please determine and give the amino acid sequence of the pentapeptide. Show which end is the amino terminus and which is the carboxy terminus. How does one arrive at the solution?
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