Biochemistry: Concepts and Connections (2nd Edition)
2nd Edition
ISBN: 9780134641621
Author: Dean R. Appling, Spencer J. Anthony-Cahill, Christopher K. Mathews
Publisher: PEARSON
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Textbook Question
Chapter 2, Problem 16P
It is possible to make a buffer that functions well near pH 7 using citric acid, which contains only carboxylate groups. Explain
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It is possible to make a buffer that functions well near pH7 using citric acid, which contains only carboxylate groups. Explain.
In ion-exchange chromatography, amino acids are separated on the basis of electro-static interactions and hydrophobic interactions with the resin. The chemical structure of the resin pol-ymer with a negatively charged sulfonic acid group is shown to the right. An elution profile for the aminoacids using this resin is given below. Based on the elution profile above, explain the following:
An appropriate biochemical buffer should have charges in both its
conjugate acid and conjugate base form. This avoids the presence
of a neutral form of the buffer that might be able slowly leak into
cells. Does unmodified dimethylpiperazine meet this criterion
when buffering at pH 8?
O Yes
No
What about buffering at pH 4? D Yes
No
Chapter 2 Solutions
Biochemistry: Concepts and Connections (2nd Edition)
Ch. 2 - Suppose a chloride ion and a sodium ion are...Ch. 2 - Draw two different possible hydrogen-bonding...Ch. 2 - Prob. 3PCh. 2 - 4. What is the pH of each of the following...Ch. 2 - Prob. 5PCh. 2 - The weak acid HA is 2% ionized (dissociated) in a...Ch. 2 - 7. Calculate the pH values and draw the titration...Ch. 2 - What is the pH of the following buffer mixtures?...Ch. 2 - a. Suppose you wanted to make a buffer of exactly...Ch. 2 - Prob. 10P
Ch. 2 - You need to make a buffer whose pH is 7.0, and you...Ch. 2 - Describe the preparation of 2.00 L of 100 glycine...Ch. 2 - Carbon dioxide is dissolved in blood (pH 7.4) to...Ch. 2 - What is the molecular basis for the observation...Ch. 2 - The anno acid arginine ionizes according to the...Ch. 2 - It is possible to make a buffer that functions...Ch. 2 - A student is carrying out a biological preparation...Ch. 2 - Histidine is an amino acid with three titratable...Ch. 2 - Prob. 19PCh. 2 - A biochemical reaction takes place in a 1.00 ml...Ch. 2 - Is RNA-binding enzyme RNase A more likely to have...Ch. 2 - Consider a protein in which a negatively charged...Ch. 2 - Prob. 23PCh. 2 - Prob. 24P
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- The pl of alkaline phosphatase is 4.5; the pl of the DEAE cellulose is 10.5. We used a buffer of pH 7.4 to run a DEAE-cellulose as anion exchange column? Explain how did this work?arrow_forwardWould it be a good idea to make up a pH 4.0 sodium acetate buffer? Why or why not?arrow_forwardWe usually say that a perfect buffer has its pH equal to its pKa. Give an example of a situation in which it would be advantageous to have a buffer with a pH 0.5 unit higher than its pKa.arrow_forward
- Which of the following is TRUE if pH is higher than the pka of the buffer? O [weak acid] approximately 0 O [conjugate base] = [weak acid] [conjugate base] > [weak acid] O [conjugate base] < [weak acid]arrow_forwardplease see attachedarrow_forwardUsing DEAE-cellulose as ion exchange resin, indicate the starting and ending pH for the narrowest experimental pH range used to separate an amino acid mixture consisting of Gln, Leu and Lys Starting pH: _____ Ending pH: _____arrow_forward
- When analyzing urine from a patient with kidney disease, which organicmolecules might be detected? Explain. Which biochemical test will be the most appropriate for detection of those molecules?arrow_forwardAn amino acid mixture consisting of lysine, leucine, and glutamic acid is to be separated by ion-exchange chromatography, using a cationexchange resin at pH 3.5, with the eluting buffer at the same pH. Which of these amino acids will be eluted from the column first? Will any other treatment be needed to elute one of these amino acids from the column?arrow_forwardDefine the following:- pH- Buffer- pKaarrow_forward
- What is the purpose of a) SDS and b) beta-mercaptoethanol in the lysis buffer for proteins being prepared for denaturing SDS-PAGE? Think about the structure, bonds and charge of proteins in terms of the purpose of these components of buffers.arrow_forwardYou need to make a protein buffer of: . • • 100 mM NaCl 25 mM Tris 8 5% w/v glycerol • 2 mM DTT Your stock solutions are: 5 M NaCl • 2 M Tris 8 • 70% w/v glycerol • DTT @ 154.25 g/mol How would you make a 1L protein buffer Solution? Show your work and describe.arrow_forwardWhat is the [CH3COO−]/[CH3COOH] ratio inan acetate buffer at pH 4.00?arrow_forward
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