(a) Interpretation: Suppose you have two genetic variants of a large protein that differ only in that one contains a histidine (side chain pK a = 6.0) when the other has a valine (uncharged side chain). The better process for separation: electrophoresis or isoelectric focusing with the required reasons. Introduction: Electrophoresis is a method to separate the macromolecules based on their sizes under the influence of electric field. Gel electrophoresis is used to separate the RNA and DNA. Isoelectric focusing is a technique to separate the proteins on the basis of pI (at which pH is zero). This is used to separate two proteins of similar molecular weight having different pI.

Question

Want to see the full answer?

Answer 1

Question

Chapter 2, Problem 24P

Interpretation Introduction

(a) Interpretation:

Suppose you have two genetic variants of a large protein that differ only in that one contains a histidine (side chain pK_a = 6.0) when the other has a valine (uncharged side chain).

The better process for separation: electrophoresis or isoelectric focusing with the required reasons.

Introduction:

Electrophoresis is a method to separate the macromolecules based on their sizes under the influence of electric field. Gel electrophoresis is used to separate the RNA and DNA.

Isoelectric focusing is a technique to separate the proteins on the basis of pI (at which pH is zero). This is used to separate two proteins of similar molecular weight having different pI.

Interpretation Introduction

(b) Interpretation:

The pH that would be chosen for the separation

Introduction:

Electrophoresis is a method to separate the macromolecules based on their sizes under the influence of electric field. Gel electrophoresis is used to separate the RNA and DNA.

Isoelectric focusing is a technique to separate the proteins on the basis of pI (at which pH is zero). This is used to separate two proteins of similar molecular weight having different pI.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Students have asked these similar questions

Show work. don't give Ai generated solution....give correct solution

Biochemistry What is the process of "transamination" in either the muscles or the liver, that involves keto acid or glutamic acid? Please explain how the steps work. Thank you!

Biochemistry Please help. Thank you What is the importance of glutamic acid in the metabolism of nitrogen from amino acids? (we know therole; it’s used to remove the nitrogen from amino acids so that the remaining carbon skeleton can bebroken down by the “usual” pathways, but what is the important, unique role that only glutamicacid/glutamate can do?)

Answer 2

Question

Chapter 2, Problem 24P

Interpretation Introduction

(a) Interpretation:

Suppose you have two genetic variants of a large protein that differ only in that one contains a histidine (side chain pK_a = 6.0) when the other has a valine (uncharged side chain).

The better process for separation: electrophoresis or isoelectric focusing with the required reasons.

Introduction:

Electrophoresis is a method to separate the macromolecules based on their sizes under the influence of electric field. Gel electrophoresis is used to separate the RNA and DNA.

Isoelectric focusing is a technique to separate the proteins on the basis of pI (at which pH is zero). This is used to separate two proteins of similar molecular weight having different pI.

Interpretation Introduction

(b) Interpretation:

The pH that would be chosen for the separation

Introduction:

Electrophoresis is a method to separate the macromolecules based on their sizes under the influence of electric field. Gel electrophoresis is used to separate the RNA and DNA.

Isoelectric focusing is a technique to separate the proteins on the basis of pI (at which pH is zero). This is used to separate two proteins of similar molecular weight having different pI.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 3

Question

Chapter 2, Problem 24P

Interpretation Introduction

(a) Interpretation:

Suppose you have two genetic variants of a large protein that differ only in that one contains a histidine (side chain pK_a = 6.0) when the other has a valine (uncharged side chain).

The better process for separation: electrophoresis or isoelectric focusing with the required reasons.

Introduction:

Electrophoresis is a method to separate the macromolecules based on their sizes under the influence of electric field. Gel electrophoresis is used to separate the RNA and DNA.

Isoelectric focusing is a technique to separate the proteins on the basis of pI (at which pH is zero). This is used to separate two proteins of similar molecular weight having different pI.

Interpretation Introduction

(b) Interpretation:

The pH that would be chosen for the separation

Introduction:

Electrophoresis is a method to separate the macromolecules based on their sizes under the influence of electric field. Gel electrophoresis is used to separate the RNA and DNA.

Isoelectric focusing is a technique to separate the proteins on the basis of pI (at which pH is zero). This is used to separate two proteins of similar molecular weight having different pI.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 4

Question

Chapter 2, Problem 24P

Interpretation Introduction

(a) Interpretation:

Suppose you have two genetic variants of a large protein that differ only in that one contains a histidine (side chain pK_a = 6.0) when the other has a valine (uncharged side chain).

The better process for separation: electrophoresis or isoelectric focusing with the required reasons.

Introduction:

Electrophoresis is a method to separate the macromolecules based on their sizes under the influence of electric field. Gel electrophoresis is used to separate the RNA and DNA.

Isoelectric focusing is a technique to separate the proteins on the basis of pI (at which pH is zero). This is used to separate two proteins of similar molecular weight having different pI.

Interpretation Introduction

(b) Interpretation:

The pH that would be chosen for the separation

Introduction:

Electrophoresis is a method to separate the macromolecules based on their sizes under the influence of electric field. Gel electrophoresis is used to separate the RNA and DNA.

Isoelectric focusing is a technique to separate the proteins on the basis of pI (at which pH is zero). This is used to separate two proteins of similar molecular weight having different pI.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 5

Chapter 2, Problem 24P

Interpretation Introduction

(a) Interpretation:

Suppose you have two genetic variants of a large protein that differ only in that one contains a histidine (side chain pK_a = 6.0) when the other has a valine (uncharged side chain).

The better process for separation: electrophoresis or isoelectric focusing with the required reasons.

Introduction:

Electrophoresis is a method to separate the macromolecules based on their sizes under the influence of electric field. Gel electrophoresis is used to separate the RNA and DNA.

Isoelectric focusing is a technique to separate the proteins on the basis of pI (at which pH is zero). This is used to separate two proteins of similar molecular weight having different pI.

Interpretation Introduction

(b) Interpretation:

The pH that would be chosen for the separation

Introduction:

Electrophoresis is a method to separate the macromolecules based on their sizes under the influence of electric field. Gel electrophoresis is used to separate the RNA and DNA.

Isoelectric focusing is a technique to separate the proteins on the basis of pI (at which pH is zero). This is used to separate two proteins of similar molecular weight having different pI.

Answer 6

Chapter 2, Problem 24P

Interpretation Introduction

(a) Interpretation:

Suppose you have two genetic variants of a large protein that differ only in that one contains a histidine (side chain pK_a = 6.0) when the other has a valine (uncharged side chain).

The better process for separation: electrophoresis or isoelectric focusing with the required reasons.

Introduction:

Electrophoresis is a method to separate the macromolecules based on their sizes under the influence of electric field. Gel electrophoresis is used to separate the RNA and DNA.

Isoelectric focusing is a technique to separate the proteins on the basis of pI (at which pH is zero). This is used to separate two proteins of similar molecular weight having different pI.

Answer 7

Chapter 2, Problem 24P

Interpretation Introduction

(a) Interpretation:

Suppose you have two genetic variants of a large protein that differ only in that one contains a histidine (side chain pK_a = 6.0) when the other has a valine (uncharged side chain).

The better process for separation: electrophoresis or isoelectric focusing with the required reasons.

Introduction:

Electrophoresis is a method to separate the macromolecules based on their sizes under the influence of electric field. Gel electrophoresis is used to separate the RNA and DNA.

Isoelectric focusing is a technique to separate the proteins on the basis of pI (at which pH is zero). This is used to separate two proteins of similar molecular weight having different pI.

Answer 8

Interpretation Introduction

(b) Interpretation:

The pH that would be chosen for the separation

Introduction:

Electrophoresis is a method to separate the macromolecules based on their sizes under the influence of electric field. Gel electrophoresis is used to separate the RNA and DNA.

Isoelectric focusing is a technique to separate the proteins on the basis of pI (at which pH is zero). This is used to separate two proteins of similar molecular weight having different pI.

Answer 9

Interpretation Introduction

(b) Interpretation:

The pH that would be chosen for the separation

Introduction:

Electrophoresis is a method to separate the macromolecules based on their sizes under the influence of electric field. Gel electrophoresis is used to separate the RNA and DNA.

Isoelectric focusing is a technique to separate the proteins on the basis of pI (at which pH is zero). This is used to separate two proteins of similar molecular weight having different pI.

Answer 10

Expert Solution & Answer

Answer 11

Expert Solution & Answer

Answer 12

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 13

Ch. 2 - Suppose a chloride ion and a sodium ion are...Ch. 2 - Draw two different possible hydrogen-bonding...Ch. 2 - Prob. 3P Ch. 2 - 4. What is the pH of each of the following...Ch. 2 - Prob. 5P Ch. 2 - The weak acid HA is 2% ionized (dissociated) in a...Ch. 2 - 7. Calculate the pH values and draw the titration...Ch. 2 - What is the pH of the following buffer mixtures?...Ch. 2 - a. Suppose you wanted to make a buffer of exactly...Ch. 2 - Prob. 10P

Want to see the full answer?

Chapter 2 Solutions