The effect in the rate of an enzyme-catalysed reaction should be determined if temperature is raised from 37°C to 70°C for an enzyme that has its maximum activity at body temperature ( about 37°C ) . Concept Introduction: Enzyme: It is a protein or a molecule which can act as a catalyst for a biological reaction. Does not affect the equilibrium point of the reaction. Active site of the enzyme is the region where the reaction takes place. Enzyme’s activity can be specific which means the activity is limited to a certain substrate and a certain type of reaction and it is referred to as specificity of the enzyme. Factors affecting enzyme activity: Substrate concentration Enzyme concentration Temperature pH

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Answer 1

Question

Chapter 19, Problem 19.56AP

(a)

Interpretation Introduction

Interpretation:

The effect in the rate of an enzyme-catalysed reaction should be determined if temperature is raised from 37°C to 70°C for an enzyme that has its maximum activity at body temperature (about 37°C).

Concept Introduction:

Enzyme:

It is a protein or a molecule which can act as a catalyst for a biological reaction.

Does not affect the equilibrium point of the reaction.

Active site of the enzyme is the region where the reaction takes place.

Enzyme’s activity can be specific which means the activity is limited to a certain substrate and a certain type of reaction and it is referred to as specificity of the enzyme.

Factors affecting enzyme activity:

Substrate concentration

Enzyme concentration

Temperature

pH

(b)

Interpretation Introduction

Interpretation:

The effect in the rate of an enzyme-catalysed reaction should be determined if the pH is lowered from 7 to 3.

Concept Introduction:

Enzyme:

It is a protein or a molecule which can act as a catalyst for a biological reaction.

Does not affect the equilibrium point of the reaction.

Active site of the enzyme is the region where the reaction takes place.

Enzyme’s activity can be specific which means the activity is limited to a certain substrate and a certain type of reaction and it is referred to as specificity of the enzyme.

Factors affecting enzyme activity:

Substrate concentration

Enzyme concentration

Temperature

pH

(c)

Interpretation Introduction

Interpretation:

The effect in the rate of an enzyme-catalysed reaction should be determined if an organic solvent like methanol is added.

Concept Introduction:

Enzyme:

It is a protein or a molecule which can act as a catalyst for a biological reaction.

Does not affect the equilibrium point of the reaction.

Active site of the enzyme is the region where the reaction takes place.

Enzyme’s activity can be specific which means the activity is limited to a certain substrate and a certain type of reaction and it is referred to as specificity of the enzyme.

Factors affecting enzyme activity:

Substrate concentration

Enzyme concentration

Temperature

pH

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The beta-lactamase hydrolyzes the lactam-ring in penicillin. Describe the mechanism of hydrolysis, insuring to include the involvement of S, D, & K in the reaction sequence. Please help

To map the active site of beta-lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine. Why doesn't D in this hexapeptide not participate in the hydrolysis of the beta-lactam ring even though S, K, and D are involved in the catalyst?

To map the active site of -lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine. Using the experimental results described above derive the primary sequence of the active site hexapeptide. Please help!

Answer 2

Question

Chapter 19, Problem 19.56AP

(a)

Interpretation Introduction

Interpretation:

The effect in the rate of an enzyme-catalysed reaction should be determined if temperature is raised from 37°C to 70°C for an enzyme that has its maximum activity at body temperature (about 37°C).

Concept Introduction:

Enzyme:

It is a protein or a molecule which can act as a catalyst for a biological reaction.

Does not affect the equilibrium point of the reaction.

Active site of the enzyme is the region where the reaction takes place.

Enzyme’s activity can be specific which means the activity is limited to a certain substrate and a certain type of reaction and it is referred to as specificity of the enzyme.

Factors affecting enzyme activity:

Substrate concentration

Enzyme concentration

Temperature

pH

(b)

Interpretation Introduction

Interpretation:

The effect in the rate of an enzyme-catalysed reaction should be determined if the pH is lowered from 7 to 3.

Concept Introduction:

Enzyme:

It is a protein or a molecule which can act as a catalyst for a biological reaction.

Does not affect the equilibrium point of the reaction.

Active site of the enzyme is the region where the reaction takes place.

Enzyme’s activity can be specific which means the activity is limited to a certain substrate and a certain type of reaction and it is referred to as specificity of the enzyme.

Factors affecting enzyme activity:

Substrate concentration

Enzyme concentration

Temperature

pH

(c)

Interpretation Introduction

Interpretation:

The effect in the rate of an enzyme-catalysed reaction should be determined if an organic solvent like methanol is added.

Concept Introduction:

Enzyme:

It is a protein or a molecule which can act as a catalyst for a biological reaction.

Does not affect the equilibrium point of the reaction.

Active site of the enzyme is the region where the reaction takes place.

Enzyme’s activity can be specific which means the activity is limited to a certain substrate and a certain type of reaction and it is referred to as specificity of the enzyme.

Factors affecting enzyme activity:

Substrate concentration

Enzyme concentration

Temperature

pH

Expert Solution & Answer

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Answer 3

Question

Chapter 19, Problem 19.56AP

(a)

Interpretation Introduction

Interpretation:

The effect in the rate of an enzyme-catalysed reaction should be determined if temperature is raised from 37°C to 70°C for an enzyme that has its maximum activity at body temperature (about 37°C).

Concept Introduction:

Enzyme:

It is a protein or a molecule which can act as a catalyst for a biological reaction.

Does not affect the equilibrium point of the reaction.

Active site of the enzyme is the region where the reaction takes place.

Enzyme’s activity can be specific which means the activity is limited to a certain substrate and a certain type of reaction and it is referred to as specificity of the enzyme.

Factors affecting enzyme activity:

Substrate concentration

Enzyme concentration

Temperature

pH

(b)

Interpretation Introduction

Interpretation:

The effect in the rate of an enzyme-catalysed reaction should be determined if the pH is lowered from 7 to 3.

Concept Introduction:

Enzyme:

It is a protein or a molecule which can act as a catalyst for a biological reaction.

Does not affect the equilibrium point of the reaction.

Active site of the enzyme is the region where the reaction takes place.

Enzyme’s activity can be specific which means the activity is limited to a certain substrate and a certain type of reaction and it is referred to as specificity of the enzyme.

Factors affecting enzyme activity:

Substrate concentration

Enzyme concentration

Temperature

pH

(c)

Interpretation Introduction

Interpretation:

The effect in the rate of an enzyme-catalysed reaction should be determined if an organic solvent like methanol is added.

Concept Introduction:

Enzyme:

It is a protein or a molecule which can act as a catalyst for a biological reaction.

Does not affect the equilibrium point of the reaction.

Active site of the enzyme is the region where the reaction takes place.

Enzyme’s activity can be specific which means the activity is limited to a certain substrate and a certain type of reaction and it is referred to as specificity of the enzyme.

Factors affecting enzyme activity:

Substrate concentration

Enzyme concentration

Temperature

pH

Expert Solution & Answer

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Check out a sample textbook solution

See solution

Answer 4

Question

Chapter 19, Problem 19.56AP

(a)

Interpretation Introduction

Interpretation:

The effect in the rate of an enzyme-catalysed reaction should be determined if temperature is raised from 37°C to 70°C for an enzyme that has its maximum activity at body temperature (about 37°C).

Concept Introduction:

Enzyme:

It is a protein or a molecule which can act as a catalyst for a biological reaction.

Does not affect the equilibrium point of the reaction.

Active site of the enzyme is the region where the reaction takes place.

Enzyme’s activity can be specific which means the activity is limited to a certain substrate and a certain type of reaction and it is referred to as specificity of the enzyme.

Factors affecting enzyme activity:

Substrate concentration

Enzyme concentration

Temperature

pH

(b)

Interpretation Introduction

Interpretation:

The effect in the rate of an enzyme-catalysed reaction should be determined if the pH is lowered from 7 to 3.

Concept Introduction:

Enzyme:

It is a protein or a molecule which can act as a catalyst for a biological reaction.

Does not affect the equilibrium point of the reaction.

Active site of the enzyme is the region where the reaction takes place.

Enzyme’s activity can be specific which means the activity is limited to a certain substrate and a certain type of reaction and it is referred to as specificity of the enzyme.

Factors affecting enzyme activity:

Substrate concentration

Enzyme concentration

Temperature

pH

(c)

Interpretation Introduction

Interpretation:

The effect in the rate of an enzyme-catalysed reaction should be determined if an organic solvent like methanol is added.

Concept Introduction:

Enzyme:

It is a protein or a molecule which can act as a catalyst for a biological reaction.

Does not affect the equilibrium point of the reaction.

Active site of the enzyme is the region where the reaction takes place.

Enzyme’s activity can be specific which means the activity is limited to a certain substrate and a certain type of reaction and it is referred to as specificity of the enzyme.

Factors affecting enzyme activity:

Substrate concentration

Enzyme concentration

Temperature

pH

Expert Solution & Answer

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Check out a sample textbook solution

See solution

Answer 5

Chapter 19, Problem 19.56AP

(a)

Interpretation Introduction

Interpretation:

The effect in the rate of an enzyme-catalysed reaction should be determined if temperature is raised from 37°C to 70°C for an enzyme that has its maximum activity at body temperature (about 37°C).

Concept Introduction:

Enzyme:

It is a protein or a molecule which can act as a catalyst for a biological reaction.

Does not affect the equilibrium point of the reaction.

Active site of the enzyme is the region where the reaction takes place.

Enzyme’s activity can be specific which means the activity is limited to a certain substrate and a certain type of reaction and it is referred to as specificity of the enzyme.

Factors affecting enzyme activity:

Substrate concentration

Enzyme concentration

Temperature

pH

(b)

Interpretation Introduction

Interpretation:

The effect in the rate of an enzyme-catalysed reaction should be determined if the pH is lowered from 7 to 3.

Concept Introduction:

Enzyme:

It is a protein or a molecule which can act as a catalyst for a biological reaction.

Does not affect the equilibrium point of the reaction.

Active site of the enzyme is the region where the reaction takes place.

Enzyme’s activity can be specific which means the activity is limited to a certain substrate and a certain type of reaction and it is referred to as specificity of the enzyme.

Factors affecting enzyme activity:

Substrate concentration

Enzyme concentration

Temperature

pH

(c)

Interpretation Introduction

Interpretation:

The effect in the rate of an enzyme-catalysed reaction should be determined if an organic solvent like methanol is added.

Concept Introduction:

Enzyme:

It is a protein or a molecule which can act as a catalyst for a biological reaction.

Does not affect the equilibrium point of the reaction.

Active site of the enzyme is the region where the reaction takes place.

Enzyme’s activity can be specific which means the activity is limited to a certain substrate and a certain type of reaction and it is referred to as specificity of the enzyme.

Factors affecting enzyme activity:

Substrate concentration

Enzyme concentration

Temperature

pH

Answer 6

Chapter 19, Problem 19.56AP

(a)

Interpretation Introduction

Interpretation:

The effect in the rate of an enzyme-catalysed reaction should be determined if temperature is raised from 37°C to 70°C for an enzyme that has its maximum activity at body temperature (about 37°C).

Concept Introduction:

Enzyme:

It is a protein or a molecule which can act as a catalyst for a biological reaction.

Does not affect the equilibrium point of the reaction.

Active site of the enzyme is the region where the reaction takes place.

Enzyme’s activity can be specific which means the activity is limited to a certain substrate and a certain type of reaction and it is referred to as specificity of the enzyme.

Factors affecting enzyme activity:

Substrate concentration

Enzyme concentration

Temperature

pH

Answer 7

Chapter 19, Problem 19.56AP

(a)

Interpretation Introduction

Interpretation:

The effect in the rate of an enzyme-catalysed reaction should be determined if temperature is raised from 37°C to 70°C for an enzyme that has its maximum activity at body temperature (about 37°C).

Concept Introduction:

Enzyme:

It is a protein or a molecule which can act as a catalyst for a biological reaction.

Does not affect the equilibrium point of the reaction.

Active site of the enzyme is the region where the reaction takes place.

Enzyme’s activity can be specific which means the activity is limited to a certain substrate and a certain type of reaction and it is referred to as specificity of the enzyme.

Factors affecting enzyme activity:

Substrate concentration

Enzyme concentration

Temperature

pH

Answer 8

(b)

Interpretation Introduction

Interpretation:

The effect in the rate of an enzyme-catalysed reaction should be determined if the pH is lowered from 7 to 3.

Concept Introduction:

Enzyme:

It is a protein or a molecule which can act as a catalyst for a biological reaction.

Does not affect the equilibrium point of the reaction.

Active site of the enzyme is the region where the reaction takes place.

Enzyme’s activity can be specific which means the activity is limited to a certain substrate and a certain type of reaction and it is referred to as specificity of the enzyme.

Factors affecting enzyme activity:

Substrate concentration

Enzyme concentration

Temperature

pH

Answer 9

(b)

Interpretation Introduction

Interpretation:

The effect in the rate of an enzyme-catalysed reaction should be determined if the pH is lowered from 7 to 3.

Concept Introduction:

Enzyme:

It is a protein or a molecule which can act as a catalyst for a biological reaction.

Does not affect the equilibrium point of the reaction.

Active site of the enzyme is the region where the reaction takes place.

Enzyme’s activity can be specific which means the activity is limited to a certain substrate and a certain type of reaction and it is referred to as specificity of the enzyme.

Factors affecting enzyme activity:

Substrate concentration

Enzyme concentration

Temperature

pH

Answer 10

(c)

Interpretation Introduction

Interpretation:

The effect in the rate of an enzyme-catalysed reaction should be determined if an organic solvent like methanol is added.

Concept Introduction:

Enzyme:

It is a protein or a molecule which can act as a catalyst for a biological reaction.

Does not affect the equilibrium point of the reaction.

Active site of the enzyme is the region where the reaction takes place.

Enzyme’s activity can be specific which means the activity is limited to a certain substrate and a certain type of reaction and it is referred to as specificity of the enzyme.

Factors affecting enzyme activity:

Substrate concentration

Enzyme concentration

Temperature

pH

Answer 11

(c)

Interpretation Introduction

Interpretation:

The effect in the rate of an enzyme-catalysed reaction should be determined if an organic solvent like methanol is added.

Concept Introduction:

Enzyme:

It is a protein or a molecule which can act as a catalyst for a biological reaction.

Does not affect the equilibrium point of the reaction.

Active site of the enzyme is the region where the reaction takes place.

Enzyme’s activity can be specific which means the activity is limited to a certain substrate and a certain type of reaction and it is referred to as specificity of the enzyme.

Factors affecting enzyme activity:

Substrate concentration

Enzyme concentration

Temperature

pH

Answer 12

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Answer 13

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Answer 14

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Answer 15

Ch. 19.1 - Prob. 19.1P Ch. 19.1 - The enzyme LDH converts lactate to pyruvate. In...Ch. 19.2 - The cofactors NAD+, Cu2+, Zn2+, coenzyme A, FAD,...Ch. 19.3 - Describe the reactions that you would expect these...Ch. 19.3 - Prob. 19.5P Ch. 19.3 - Prob. 19.6P Ch. 19.3 - Prob. 19.7P Ch. 19.3 - Prob. 19.8P Ch. 19.4 - Prob. 19.9KCP Ch. 19.5 - Prob. 19.10KCP

Solution Summary: The author explains that the rate of an enzyme-catalysed reaction should be determined if the temperature is lowered from 7 to 3.

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Chapter 19 Solutions