Biochemistry
9th Edition
ISBN: 9781305961135
Author: Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher: Cengage Learning
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Chapter 18, Problem 20RE
RECALL What reactions in this chapter require acetyl-CoA or biotin?
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The beta-lactamase hydrolyzes the lactam-ring in penicillin. Describe the mechanism
of hydrolysis, insuring to include the involvement of S, D, & K in the reaction sequence. Please help
To map the active site of beta-lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine.
Why doesn't D in this hexapeptide not participate in the hydrolysis of the beta-lactam ring even though S, K, and D are involved in the catalyst?
To map the active site of -lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine.
Using the experimental results described above derive the primary sequence of the active site hexapeptide. Please help!
Chapter 18 Solutions
Biochemistry
Ch. 18 - RECALL Why is it essential that the mechanisms...Ch. 18 - RECALL How does phosphorolysis differ from...Ch. 18 - RECALL Why is it advantageous that breakdown of...Ch. 18 - RECALL Briefly outline the role of UDPG in...Ch. 18 - RECALL Name two control mechanisms that play a...Ch. 18 - REFLECT AND APPLY Does the net gain of ATP in...Ch. 18 - REFLECT AND APPLY In metabolism,...Ch. 18 - Prob. 8RECh. 18 - BIOCHEMICAL CONNECTIONS You are planning to go on...Ch. 18 - BIOCHEMICAL CONNECTIONS Would eating candy bars,...
Ch. 18 - Prob. 11RECh. 18 - Prob. 12RECh. 18 - REFLECT AND APPLY A researcher claims to have...Ch. 18 - REFLECT AND APPLY What is the source of the energy...Ch. 18 - Prob. 15RECh. 18 - Prob. 16RECh. 18 - Prob. 17RECh. 18 - Prob. 18RECh. 18 - Prob. 19RECh. 18 - RECALL What reactions in this chapter require...Ch. 18 - RECALL Which steps of glycolysis are irreversible?...Ch. 18 - RECALL What is the role of biotin in...Ch. 18 - RECALL How does the role of glucose-6-phosphate in...Ch. 18 - REFLECT AND APPLY Avidin, a protein found in egg...Ch. 18 - REFLECT AND APPLY How does the hydrolysis of...Ch. 18 - Prob. 26RECh. 18 - Prob. 27RECh. 18 - Prob. 28RECh. 18 - Prob. 29RECh. 18 - Prob. 30RECh. 18 - Prob. 31RECh. 18 - REFLECT AND APPLY How can different time scales...Ch. 18 - REFLECT AND APPLY How do the control mechanisms in...Ch. 18 - Prob. 34RECh. 18 - Prob. 35RECh. 18 - Prob. 36RECh. 18 - REFLECT AND APPLY How can the synthesis and...Ch. 18 - REFLECT AND APPLY How is it advantageous for...Ch. 18 - Prob. 39RECh. 18 - Prob. 40RECh. 18 - Prob. 41RECh. 18 - Prob. 42RECh. 18 - RECALL What roles do glucagon and epinephrine play...Ch. 18 - Prob. 44RECh. 18 - RECALL List three differences in structure or...Ch. 18 - RECALL What are four possible metabolic fates of...Ch. 18 - BIOCHEMICAL CONNECTIONS What is the connection...Ch. 18 - Prob. 48RECh. 18 - RECALL What is a major difference between...Ch. 18 - Prob. 50RECh. 18 - Prob. 51RECh. 18 - Prob. 52RECh. 18 - REFLECT AND APPLY Suggest a reason why a different...Ch. 18 - REFLECT AND APPLY Explain how the pentose...Ch. 18 - REFLECT AND APPLY Why is it reasonable to expect...Ch. 18 - REFLECT AND APPLY How would it affect the...
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