Chapter 14.SE, Problem 61AP

Interpretation Introduction

Interpretation:

The concentration of the ergosterol solution whose absorbance A= 0.065 with a sample pathlength l = 1.00 cm is to be calculated if ergosterol has a UV absorption maxima of 282 nm and mole absorptivity ε = 11,900.

Concept introduction:

The molar absorptivity, ε, is given by where A is absorbance, C is the concentration of the solution in moles/lit and l is the sample path length in cm.

To calculate:

The concentration of the ergosterol solution whose absorbance A= 0.065 with a sample pathlength l = 1.00 cm, if ergosterol has a UV absorption maxima of 282 nm and mole absorptivity ε = 11,900.