Biochemistry: The Molecular Basis of Life
6th Edition
ISBN: 9780190209896
Author: Trudy McKee, James R. McKee
Publisher: Oxford University Press
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Chapter 13, Problem 7RQ
Summary Introduction
To review:
The definition of the following terminologies:
a. Wavelength
b. Chromophore
c. Fluorescence
d. Radiationless decay
e. Resonance energy transfer
Introduction:
The
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Fura-2 Fluorescence (Arbitrary Unit)
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[Ca2+]=2970nM, 25°C
[Ca2+] 2970nM, 4°C
[Ca2+]=0.9nM, 25°C
[Ca2+] = 0.9nM, 4°C
0
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Wavelength (nm)
←
<
The figure on the LHS shows the excitation spectra of Fura-2 (Em
= 510 nm) in 2 solutions with two different Ca2+ ion concentration
as indicated. Except for temperature, the setting for excitation &
signal acquisition was identical.<
ப
a) The unit in Y-axis is arbitrary (unspecified). Why? <
<
b) Compare & contrast the excitation wavelength of the Isosbestic
Point of Fura-2 at 25 °C & 4 °C. Give a possible reason for the
discrepancy. <
c) The fluorescence intensity at 25 °C & 4 °C are different. Explain
why with the concept of electronic configuration. <
Chapter 13 Solutions
Biochemistry: The Molecular Basis of Life
Ch. 13 - Prob. 1QCh. 13 - Prob. 2QCh. 13 - Prob. 3QCh. 13 - Prob. 4QCh. 13 - Prob. 5QCh. 13 - Prob. 6QCh. 13 - Prob. 7QCh. 13 - Prob. 1RQCh. 13 - Prob. 2RQCh. 13 - Prob. 3RQ
Ch. 13 - Prob. 4RQCh. 13 - Prob. 5RQCh. 13 - Prob. 6RQCh. 13 - Prob. 7RQCh. 13 - Prob. 8RQCh. 13 - Prob. 9RQCh. 13 - Prob. 10RQCh. 13 - Prob. 11RQCh. 13 - Prob. 12RQCh. 13 - Prob. 13RQCh. 13 - Prob. 14RQCh. 13 - Prob. 15RQCh. 13 - Prob. 16RQCh. 13 - Prob. 17RQCh. 13 - Prob. 18RQCh. 13 - Prob. 19RQCh. 13 - Prob. 20RQCh. 13 - Prob. 21RQCh. 13 - Prob. 22RQCh. 13 - Prob. 23RQCh. 13 - Prob. 24RQCh. 13 - Prob. 25RQCh. 13 - Prob. 26RQCh. 13 - Prob. 27RQCh. 13 - Prob. 28RQCh. 13 - Prob. 29RQCh. 13 - Prob. 30RQCh. 13 - Prob. 31RQCh. 13 - Prob. 32RQCh. 13 - Prob. 33RQCh. 13 - Prob. 34RQCh. 13 - Prob. 35RQCh. 13 - Prob. 36RQCh. 13 - Prob. 37RQCh. 13 - Prob. 38RQCh. 13 - Prob. 39RQCh. 13 - Prob. 40RQCh. 13 - Prob. 41FBCh. 13 - Prob. 42FBCh. 13 - Prob. 43FBCh. 13 - Prob. 44FBCh. 13 - Prob. 45FBCh. 13 - Prob. 46FBCh. 13 - Prob. 47FBCh. 13 - Prob. 48FBCh. 13 - Prob. 49FBCh. 13 - Prob. 50FBCh. 13 - Prob. 51SACh. 13 - Prob. 52SACh. 13 - Prob. 53SACh. 13 - Prob. 54SACh. 13 - Prob. 55SACh. 13 - Prob. 56TQCh. 13 - Prob. 57TQCh. 13 - Prob. 58TQCh. 13 - Prob. 59TQCh. 13 - Prob. 60TQCh. 13 - Prob. 61TQCh. 13 - Prob. 62TQCh. 13 - Prob. 63TQCh. 13 - Prob. 64TQCh. 13 - Prob. 65TQCh. 13 - Prob. 66TQCh. 13 - Prob. 67TQCh. 13 - Prob. 68TQCh. 13 - Prob. 69TQCh. 13 - Prob. 70TQCh. 13 - Prob. 71TQCh. 13 - Prob. 72TQCh. 13 - Prob. 73TQCh. 13 - Prob. 74TQ
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