
Biochemistry: The Molecular Basis of Life
6th Edition
ISBN: 9780190209896
Author: Trudy McKee, James R. McKee
Publisher: Oxford University Press
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Question
Chapter 13, Problem 5Q
Summary Introduction
To review:
The role of the following during photosynthesis:
a. Plastocyanin
b. β-Carotene
c. Ferredoxin
d. Plastoquinone
e. Pheophytina
f. Lutein
Introduction:
Photosynthesis is the process of producing food in the form of sugar by green plants, cyanobacteri, and some Protists. It is a light-driven biochemical mechanism as it requiresenergy from sunlight to synthesize ATP (adenosine triphosphate) and NADPH (nicotinamide adenine dinucleotide phosphate) in the form of energy. In this proces, sarbon dioxide is incorporated into organic molecule, glucose. This process takes place inside the photosystems (membrane-bound protein complex in chloroplast) in plants.
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The beta-lactamase hydrolyzes the lactam-ring in penicillin. Describe the mechanism
of hydrolysis, insuring to include the involvement of S, D, & K in the reaction sequence. Please help
To map the active site of beta-lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine.
Why doesn't D in this hexapeptide not participate in the hydrolysis of the beta-lactam ring even though S, K, and D are involved in the catalyst?
To map the active site of -lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine.
Using the experimental results described above derive the primary sequence of the active site hexapeptide. Please help!
Chapter 13 Solutions
Biochemistry: The Molecular Basis of Life
Ch. 13 - Prob. 1QCh. 13 - Prob. 2QCh. 13 - Prob. 3QCh. 13 - Prob. 4QCh. 13 - Prob. 5QCh. 13 - Prob. 6QCh. 13 - Prob. 7QCh. 13 - Prob. 1RQCh. 13 - Prob. 2RQCh. 13 - Prob. 3RQ
Ch. 13 - Prob. 4RQCh. 13 - Prob. 5RQCh. 13 - Prob. 6RQCh. 13 - Prob. 7RQCh. 13 - Prob. 8RQCh. 13 - Prob. 9RQCh. 13 - Prob. 10RQCh. 13 - Prob. 11RQCh. 13 - Prob. 12RQCh. 13 - Prob. 13RQCh. 13 - Prob. 14RQCh. 13 - Prob. 15RQCh. 13 - Prob. 16RQCh. 13 - Prob. 17RQCh. 13 - Prob. 18RQCh. 13 - Prob. 19RQCh. 13 - Prob. 20RQCh. 13 - Prob. 21RQCh. 13 - Prob. 22RQCh. 13 - Prob. 23RQCh. 13 - Prob. 24RQCh. 13 - Prob. 25RQCh. 13 - Prob. 26RQCh. 13 - Prob. 27RQCh. 13 - Prob. 28RQCh. 13 - Prob. 29RQCh. 13 - Prob. 30RQCh. 13 - Prob. 31RQCh. 13 - Prob. 32RQCh. 13 - Prob. 33RQCh. 13 - Prob. 34RQCh. 13 - Prob. 35RQCh. 13 - Prob. 36RQCh. 13 - Prob. 37RQCh. 13 - Prob. 38RQCh. 13 - Prob. 39RQCh. 13 - Prob. 40RQCh. 13 - Prob. 41FBCh. 13 - Prob. 42FBCh. 13 - Prob. 43FBCh. 13 - Prob. 44FBCh. 13 - Prob. 45FBCh. 13 - Prob. 46FBCh. 13 - Prob. 47FBCh. 13 - Prob. 48FBCh. 13 - Prob. 49FBCh. 13 - Prob. 50FBCh. 13 - Prob. 51SACh. 13 - Prob. 52SACh. 13 - Prob. 53SACh. 13 - Prob. 54SACh. 13 - Prob. 55SACh. 13 - Prob. 56TQCh. 13 - Prob. 57TQCh. 13 - Prob. 58TQCh. 13 - Prob. 59TQCh. 13 - Prob. 60TQCh. 13 - Prob. 61TQCh. 13 - Prob. 62TQCh. 13 - Prob. 63TQCh. 13 - Prob. 64TQCh. 13 - Prob. 65TQCh. 13 - Prob. 66TQCh. 13 - Prob. 67TQCh. 13 - Prob. 68TQCh. 13 - Prob. 69TQCh. 13 - Prob. 70TQCh. 13 - Prob. 71TQCh. 13 - Prob. 72TQCh. 13 - Prob. 73TQCh. 13 - Prob. 74TQ
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Photosynthesis & Respiration | Reactions | Chemistry | FuseSchool; Author: FuseSchool - Global Education;https://www.youtube.com/watch?v=3XIyweZg6Sw;License: Standard YouTube License, CC-BY