Biochemistry: The Molecular Basis of Life
6th Edition
ISBN: 9780190209896
Author: Trudy McKee, James R. McKee
Publisher: Oxford University Press
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Chapter 13, Problem 56TQ
Summary Introduction
To review:
The use of biofuel over the use of fossil fuel.
Introduction:
Fossil fuels are buried geological combustible deposits of organic matter. These include coal, crude oil, and others. These fuels have been exploited over the years for various purposes. New means for the purpose of production of energy have been introduced. These are biofuelsthat are producedwith the help of biological processes.
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The beta-lactamase hydrolyzes the lactam-ring in penicillin. Describe the mechanism
of hydrolysis, insuring to include the involvement of S, D, & K in the reaction sequence. Please help
To map the active site of beta-lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine.
Why doesn't D in this hexapeptide not participate in the hydrolysis of the beta-lactam ring even though S, K, and D are involved in the catalyst?
To map the active site of -lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine.
Using the experimental results described above derive the primary sequence of the active site hexapeptide. Please help!
Chapter 13 Solutions
Biochemistry: The Molecular Basis of Life
Ch. 13 - Prob. 1QCh. 13 - Prob. 2QCh. 13 - Prob. 3QCh. 13 - Prob. 4QCh. 13 - Prob. 5QCh. 13 - Prob. 6QCh. 13 - Prob. 7QCh. 13 - Prob. 1RQCh. 13 - Prob. 2RQCh. 13 - Prob. 3RQ
Ch. 13 - Prob. 4RQCh. 13 - Prob. 5RQCh. 13 - Prob. 6RQCh. 13 - Prob. 7RQCh. 13 - Prob. 8RQCh. 13 - Prob. 9RQCh. 13 - Prob. 10RQCh. 13 - Prob. 11RQCh. 13 - Prob. 12RQCh. 13 - Prob. 13RQCh. 13 - Prob. 14RQCh. 13 - Prob. 15RQCh. 13 - Prob. 16RQCh. 13 - Prob. 17RQCh. 13 - Prob. 18RQCh. 13 - Prob. 19RQCh. 13 - Prob. 20RQCh. 13 - Prob. 21RQCh. 13 - Prob. 22RQCh. 13 - Prob. 23RQCh. 13 - Prob. 24RQCh. 13 - Prob. 25RQCh. 13 - Prob. 26RQCh. 13 - Prob. 27RQCh. 13 - Prob. 28RQCh. 13 - Prob. 29RQCh. 13 - Prob. 30RQCh. 13 - Prob. 31RQCh. 13 - Prob. 32RQCh. 13 - Prob. 33RQCh. 13 - Prob. 34RQCh. 13 - Prob. 35RQCh. 13 - Prob. 36RQCh. 13 - Prob. 37RQCh. 13 - Prob. 38RQCh. 13 - Prob. 39RQCh. 13 - Prob. 40RQCh. 13 - Prob. 41FBCh. 13 - Prob. 42FBCh. 13 - Prob. 43FBCh. 13 - Prob. 44FBCh. 13 - Prob. 45FBCh. 13 - Prob. 46FBCh. 13 - Prob. 47FBCh. 13 - Prob. 48FBCh. 13 - Prob. 49FBCh. 13 - Prob. 50FBCh. 13 - Prob. 51SACh. 13 - Prob. 52SACh. 13 - Prob. 53SACh. 13 - Prob. 54SACh. 13 - Prob. 55SACh. 13 - Prob. 56TQCh. 13 - Prob. 57TQCh. 13 - Prob. 58TQCh. 13 - Prob. 59TQCh. 13 - Prob. 60TQCh. 13 - Prob. 61TQCh. 13 - Prob. 62TQCh. 13 - Prob. 63TQCh. 13 - Prob. 64TQCh. 13 - Prob. 65TQCh. 13 - Prob. 66TQCh. 13 - Prob. 67TQCh. 13 - Prob. 68TQCh. 13 - Prob. 69TQCh. 13 - Prob. 70TQCh. 13 - Prob. 71TQCh. 13 - Prob. 72TQCh. 13 - Prob. 73TQCh. 13 - Prob. 74TQ
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