The dangers of the recombinant DNA technology are to be determined. Concept introduction: Introduction of DNA into the genome of virus and bacteria is possible. It is known as insert. Usually, insert is tagged to a plasmid, which is a small circular DNA present in bacteria. The insert grows and makes multiple copies by the process of cloning.

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Example 1: 1. Suppose an enzyme (MW = 5,000 g/mole) has a concentration of 0.05 mg/L. If the kcat is 1 x 10 s, what is the theoretical maximum reaction velocity for the enzyme? A) 1050 µM/s. B) 100 µM/s. C) 150 μM/s. D) 105 μM/s.

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Question

Chapter 13, Problem 19RE

Interpretation Introduction

Interpretation:

The dangers of the recombinant DNA technology are to be determined.

Concept introduction:

Introduction of DNA into the genome of virus and bacteria is possible. It is known as insert.

Usually, insert is tagged to a plasmid, which is a small circular DNA present in bacteria.

The insert grows and makes multiple copies by the process of cloning.

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Example 1: 1. Suppose an enzyme (MW = 5,000 g/mole) has a concentration of 0.05 mg/L. If the kcat is 1 x 10 s, what is the theoretical maximum reaction velocity for the enzyme? A) 1050 µM/s. B) 100 µM/s. C) 150 μM/s. D) 105 μM/s.

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