Biochemistry
9th Edition
ISBN: 9781305961135
Author: Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher: Cengage Learning
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Chapter 13, Problem 12RE
RECALL What are sticky ends? What is their importance in recombinant DNA technology?
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Chapter 13 Solutions
Biochemistry
Ch. 13 - RECALL What advantages does fluorescent labeling...Ch. 13 - RECALL What methods are used to visualize...Ch. 13 - REFLECT AND APPLY When proteins are separated...Ch. 13 - RECALL How does the use of restriction...Ch. 13 - RECALL What is the importance of methylation in...Ch. 13 - RECALL Why do restriction endonucleases not...Ch. 13 - Prob. 7RECh. 13 - Prob. 8RECh. 13 - RECALL What do the following have in common? MOM;...Ch. 13 - RECALL Give three examples of DNA palindromes.
Ch. 13 - RECALL What are three differences between the...Ch. 13 - RECALL What are sticky ends? What is their...Ch. 13 - RECALL What would be an advantage of using HaeIII...Ch. 13 - RECALL Describe the cloning of DNA.Ch. 13 - RECALL What vectors can be used for cloning?Ch. 13 - RECALL Describe the method you would use to test...Ch. 13 - RECALL What is blue/white screening? What is the...Ch. 13 - Prob. 18RECh. 13 - Prob. 19RECh. 13 - Prob. 20RECh. 13 - Prob. 21RECh. 13 - Prob. 22RECh. 13 - Prob. 23RECh. 13 - REFLECT AND APPLY What are the requirements for an...Ch. 13 - Prob. 25RECh. 13 - Prob. 26RECh. 13 - REFLECT AND APPLY The genes for both the a- and...Ch. 13 - REFLECT AND APPLY Outline the methods you would...Ch. 13 - Prob. 29RECh. 13 - Prob. 30RECh. 13 - Prob. 31RECh. 13 - Prob. 32RECh. 13 - RECALL Why is temperature control so important in...Ch. 13 - RECALL Why is the use of temperature-stable DNA...Ch. 13 - RECALL What are the criteria for good primers in a...Ch. 13 - REFLECT AND APPLY What difficulties arise in the...Ch. 13 - REFLECT AND APPLY Each of the following pairs of...Ch. 13 - RECALL What is qPCR?Ch. 13 - Prob. 39RECh. 13 - REFLECT AND APPLY Suppose that you are a...Ch. 13 - REFLECT AND APPLY Why is DNA evidence more useful...Ch. 13 - REFLECT AND APPLY Give the DNA sequence for the...Ch. 13 - Prob. 43RECh. 13 - Prob. 44RECh. 13 - Prob. 45RECh. 13 - Prob. 46RECh. 13 - Prob. 47RECh. 13 - RECALL Has proteomic analysis been done on...Ch. 13 - Prob. 49RECh. 13 - Prob. 50RECh. 13 - Prob. 51RECh. 13 - RECALL What are the key differences between DNA...
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- REFLECT AND APPLY What is the complete base composition of a double-stranded eukaryotic DNA that contains 22% guanine?arrow_forwardREFLECT AND APPLY Give the DNA sequence for the template strand that gives rise to the following sequence gel, prepared using the Sanger method with a radioactive label at the 5' end of the primer.arrow_forwardREFLECT AND APPLY In the MeselsonStahl experiment that established the semiconservative nature of DNA replication, the extraction method produced short fragments of DNA. What sort of results might have been obtained with longer pieces of DNA?arrow_forward
- REFLECT AND APPLY What difficulties arise in the polymerase chain reaction if there is contamination of the DNA that is to be copied?arrow_forwardREFLECT AND APPLY (a) Eukaryotic DNA replication is more complex than prokaryotic replication. Give one reason why this should be so. (b) Why might eukaryotic cells need more kinds of DNA polymerases than bacteria?arrow_forwardREFLECT AND APPLY (a) Is it biologically advantageous that DNA is stable? Why or why not? (b) Is it biologically advantageous that RNA is unstable? Why or why not?arrow_forward
- REFLECT AND APPLY Why is it more important for DNA to be replicated accurately than transcribed accurately?arrow_forwardRECALL What are the key differences between DNA microarrays and protein microarrays, and how they are used in research?arrow_forwardRECALL Do DNA-polymerase enzymes also function as exonucleases?arrow_forward
- RECALL Compare and contrast the properties of the enzymes DNA polymerase I and polymerase III from E. coli.arrow_forwardREFLECT AND APPLY When proteins are separated using native gel electrophoresis, size, shape, and charge control their rate of migration on the gel. Why does DNA separate based on size, and why do we not worry much about shape or charge?arrow_forwardRECALL What is blue/white screening? What is the key feature of a plasmid that is used for it?arrow_forward
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